Ohtsuki K
Tex Rep Biol Med. 1977;35:270-81.
Treatment of mouse L cells with homologous interferon results in the production of a protein which inhibits initiation factor activity. Characterization experiments indicate that the interferon in the preparation is responsible for the induction of an inhibitor protein. The impairment of initiation factor activity induced by interferon is specifically reversed by cAMP. An inhibitor protein may be separated from the initiation factor activity by glycerol density gradient sedimentation. The molecular weight of the inhibitor is about 50,000 dalton and sediments with protein kinase activity.
用同源干扰素处理小鼠L细胞会产生一种抑制起始因子活性的蛋白质。特性鉴定实验表明，制剂中的干扰素负责诱导一种抑制蛋白。环磷酸腺苷（cAMP）可特异性逆转干扰素诱导的起始因子活性损伤。通过甘油密度梯度沉降可将抑制蛋白与起始因子活性分离。该抑制蛋白的分子量约为50,000道尔顿，并与蛋白激酶活性一起沉降。
