Kazuń Barbara, Małaczewska Joanna, Kazuń Krzysztof, Żylińska-Urban Joanna, Siwicki Andrzej K
Department of Fish Pathology and Immunology, Stanislaw Sakowicz Inland Fisheries Institute, 10-719 Olsztyn, Poland.
Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719 Olsztyn, Poland.
J Vet Res. 2018 Dec 31;62(4):485-492. doi: 10.2478/jvetres-2018-0062. eCollection 2018 Dec.
Immune-potentiating functions of strains in the common carp were evaluated.
Fourteen days of feeding fish dry diet supplemented with the bacteria provided parameters of nonspecific humoral immunity (lysozyme, ceruloplasmin, γ-globulin, total protein levels, and serum bactericidal activity) and cellular immunity (pinocytosis, respiratory burst activity, and potential killing activity of organ phagocytes), as well as the proliferative response of organ lymphocytes stimulated with mitogens. The resistance of fish to infection with was also determined.
Dietary supplementation with had a substantial influence on the activity of organ phagocytes, especially the potential killing activity of head kidney cells. A significant increase in the proliferative activity of LPS-stimulated B lymphocytes and in the levels of γ-globulins and total protein was observed. The supplemented diet conveyed higher resistance than the control diet as the cumulative fish mortalities after infection with were 65% and 85%, respectively.
The results indicate that dietary supplementation with stimulates the antibacterial resistance of common carp and may reinforce defence against bacterial infections, but further studies need to be conducted.
评估了鲤鱼中菌株的免疫增强功能。
用添加了细菌的干饲料喂养鱼类14天，提供了非特异性体液免疫（溶菌酶、铜蓝蛋白、γ-球蛋白、总蛋白水平和血清杀菌活性）和细胞免疫（吞噬作用、呼吸爆发活性和器官吞噬细胞的潜在杀伤活性）的参数，以及用丝裂原刺激的器官淋巴细胞的增殖反应。还测定了鱼类对感染的抵抗力。
饮食中添加对器官吞噬细胞的活性有显著影响，尤其是头肾细胞的潜在杀伤活性。观察到脂多糖刺激的B淋巴细胞增殖活性以及γ-球蛋白和总蛋白水平显著增加。添加了的饲料比对照饲料具有更高的抵抗力，因为感染后的累计鱼类死亡率分别为65%和85%。
结果表明，饮食中添加可刺激鲤鱼的抗菌抵抗力，并可能增强对细菌感染的防御，但需要进一步研究。
