Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China.
J Am Chem Soc. 2011 Feb 2;133(4):686-8. doi: 10.1021/ja108788p.
Persistent-luminescence nanoparticles (PLNPs) are promising as a new generation of photoluminescent probes for detection of biomolecules and bioimaging. Here we report a fluorescence resonance energy transfer (FRET) inhibition assay for α-fetoprotein (AFP) excreted during cancer cell growth using water-soluble functionalized PLNPs based on Eu2+- and Dy3+-doped Ca1.86Mg0.14ZnSi2O7. Polyethyleneimine-coated PLNPs were conjugated with AFP-antibody-coated gold nanoparticles as a sensitive and specific persistent photoluminescence probe for detection of AFP in serum samples and imaging of AFP excreted during cancer cell growth. Such PLNPs do not contain toxic heavy metals. Their long-lasting afterglow nature allows detection and imaging without external illumination, thereby eliminating the autofluorescence and scattering light from biological matrixes encountered under in situ excitation.
持续发光纳米粒子（PLNPs）作为新一代的光致发光探针，在用于检测生物分子和生物成像方面具有广阔的应用前景。在这里，我们报告了一种基于 Eu2+-和 Dy3+-掺杂的 Ca1.86Mg0.14ZnSi2O7 的荧光共振能量转移（FRET）抑制法，用于检测癌细胞生长过程中分泌的甲胎蛋白（AFP）。水溶性功能化的 PLNPs 与 AFP-抗体包被的金纳米颗粒偶联，作为一种灵敏和特异的持续光致发光探针，用于检测血清样本中的 AFP，并对癌细胞生长过程中分泌的 AFP 进行成像。这种 PLNPs 不含有毒重金属。它们的持久余辉特性允许在没有外部照明的情况下进行检测和成像，从而消除了在原位激发下遇到的生物基质的自发荧光和散射光。
