Liu Jing-Min, Yuan Xin-Yue, Liu Hui-Lin, Cheng Dai, Wang Shuo
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University Tianjin 300071 China
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU) Beijing 100048 China.
RSC Adv. 2018 Aug 8;8(50):28414-28420. doi: 10.1039/c8ra05555f. eCollection 2018 Aug 7.
The development of and real-time analytical methods for specifically probing food-borne hazardous substances is promising for clarifying their harmful behaviors and related disease mechanisms inside the living body through investigation of their behaviors. Herein, optical nanoimaging with the ability of non-damage detection and real-time monitoring was introduced for specific recognition of aflatoxin in cellular levels and the fluorescence resonance energy transfer (FRET) protocol. Persistent luminescence nanophosphors (PLNPs) with distinct advantages of improved sensitivity and signal-to-noise ratio were employed in bioimaging as photoluminescence nanoprobes, while copper sulfide nanoparticles were utilized as the quencher. Due to their long-lasting afterglow, PLNPs do not require external illumination before imaging, effectively eliminating the scattering light and autofluorescence from the biological matrix that can occur during excitation. The proposed FRET imaging assay achieved high sensitivity and specificity as well as improved imaging resolution for the target aflatoxin present . This study will provide insights towards advanced methodology for the applications of bioimaging in food safety, and could potentially provide an advisory roadmap for bioimaging-guided exploration and mediation of food-borne hazards to human health.
开发用于特异性探测食源有害物质的实时分析方法，有望通过研究其行为来阐明它们在生物体内的有害行为及相关疾病机制。在此，引入了具有非损伤检测和实时监测能力的光学纳米成像技术，用于在细胞水平上特异性识别黄曲霉毒素，并采用了荧光共振能量转移（FRET）方案。具有提高灵敏度和信噪比等明显优势的持续发光纳米磷光体（PLNPs）被用作光致发光纳米探针用于生物成像，而硫化铜纳米颗粒则用作猝灭剂。由于其持久的余辉，PLNPs在成像前不需要外部照明，有效消除了生物基质在激发过程中可能产生的散射光和自发荧光。所提出的FRET成像分析方法对存在的目标黄曲霉毒素具有高灵敏度、高特异性以及更高的成像分辨率。本研究将为生物成像在食品安全中的应用提供先进方法的见解，并有可能为生物成像引导的食源危害对人类健康的探索和调控提供咨询路线图。
