Urogenital Research Unit. State University of Rio de Janeiro, RJ, Brazil.
Int Braz J Urol. 2013 May-Jun;39(3):424-31. doi: 10.1590/S1677-5538.IBJU.2013.03.17.
To assess the morphological changes of penile vascular structures and the corpus cavernosum area in alloxan-induced diabetic rabbits.
Twenty male rabbits (2 months old) were divided into two groups with 10 rabbits each, the control group (CG) and the diabetic group (DG). The animals from DG received an intravenous injection of alloxan (100mg/kg) to induce the diabetes. Ten weeks after the induction of diabetes, all animals were euthanized. Two fragments of the penile shaft were harvested and samples were processed and paraffin embedded. Sections (5 µm) were cut and stained for histological and immunohistochemical markers.
Nuclear protrusion toward the lumen, and cytoplasmic vacuolization were observed in the tunica intima of the dorsal artery of the penis in DG. The thicknesses of the tunica media increased significantly in DG (p = 0.0350). It was also observed a significant increase in the area of the tunica media (p = 0.0179). There was no significant change in smooth muscle cell density in the tunica media of the dorsal artery of the penis (p = 0.0855). The collagen fiber pattern of the tunica adventitia of the dorsal artery of the penis was different between the control and diabetic groups. There was a significant decrease in the area occupied by the cavernous sinuses in DG (p = 0.0013).
Alloxan-induced diabetes mellitus in rabbits promotes important changes in penile vascular structures, thereby decreasing blood supply and affecting penile hemodynamics, leading to erectile dysfunction.
评估糖尿病兔阴茎血管结构和海绵体区域的形态变化。
20 只雄性兔（2 月龄）分为两组，每组 10 只，对照组（CG）和糖尿病组（DG）。DG 组动物静脉注射链脲佐菌素（100mg/kg）诱导糖尿病。糖尿病诱导 10 周后，处死所有动物。采集阴茎干 2 段，处理标本并石蜡包埋。切片（5μm），行组织学和免疫组织化学标志物染色。
DG 组阴茎背动脉内膜出现核突向管腔、胞质空泡化。DG 组中膜厚度显著增加（p=0.0350），中膜面积显著增加（p=0.0179）。阴茎背动脉中膜平滑肌细胞密度无显著变化（p=0.0855）。DG 组阴茎背动脉外膜胶原纤维模式与对照组不同。DG 组海绵窦面积显著减少（p=0.0013）。
兔糖尿病诱导的糖尿病可引起阴茎血管结构的重要变化，从而减少血液供应，影响阴茎血液动力学，导致勃起功能障碍。
