Department of Chemistry, The University of Burdwan, Golapbag, West Bengal, India.
Phytochemistry. 2011 Feb;72(2-3):276-83. doi: 10.1016/j.phytochem.2010.11.006. Epub 2010 Dec 15.
Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance to virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study, we have analyzed xylogalactofucan- and alginic acid-containing fractions from Sphacelaria indica, a marine alga. The xylogalactofucan that has apparent molecular mass of 26±5 kDa and negative specific rotation α(32) -71° (c 0.2, H(2)O) contains, inter alia, (1→3)-linked L-fucopyranosyl and D-galactopyranosyl residues. The algin (molecular mass: 21±5kDa) contains 41% guluronic and 59% mannuronic acid residues. The 50% inhibitory concentration (IC(50)) values of these macromolecules and their chemically sulfated derivatives against herpes simplex virus type 1 (HSV-1) were in the range of 0.6-10 μg ml(-1) and they lacked cytotoxicity at concentrations up to 200 μg ml(-1). The antiviral activity was dependent on the sulfate contents of the polysaccharides. The results support the feasibility of inhibiting HSV infection by direct interaction of polysaccharides with viral particles.
许多病毒对细胞表面硫酸乙酰肝素蛋白聚糖具有亲和力，这与病毒进入具有生物学相关性。这就提出了在抗病毒治疗中应用硫酸多糖的可能性。在这项研究中，我们分析了来自海洋藻类角叉菜的木糖半乳糖醛酸和藻酸盐含量的组分。具有明显的 26±5 kDa 分子量和负比旋光度 α(32) -71°（c 0.2，H(2)O）的木糖半乳醛酸除其他外，还包含（1→3）连接的 L-岩藻糖吡喃基和 D-半乳糖吡喃基残基。藻酸（分子量：21±5kDa）含有 41%的古洛糖醛酸和 59%的甘露糖醛酸残基。这些大分子及其化学硫酸化衍生物对单纯疱疹病毒 1（HSV-1）的 50%抑制浓度（IC(50)）值在 0.6-10 μg ml(-1)范围内，在高达 200 μg ml(-1)的浓度下它们没有细胞毒性。抗病毒活性取决于多糖的硫酸化含量。结果支持了通过多糖与病毒颗粒的直接相互作用抑制 HSV 感染的可行性。
