Department of Land, Environment, Agriculture and Forestry, University of Padua, Legnaro (PD) Italy.
Mol Plant Microbe Interact. 2011 Apr;24(4):432-40. doi: 10.1094/MPMI-07-10-0157.
The ability of bacterial or fungal necrotrophs to produce enzymes capable of degrading pectin is often related to a successful initiation of the infective process. Pectin is synthesized in a highly methylesterified form and is subsequently de-esterified in muro by pectin methylesterase. De-esterification makes pectin more susceptible to the degradation by pectic enzymes such as endopolygalacturonases (endoPG) and pectate lyases secreted by necrotrophic pathogens during the first stages of infection. We show that, upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of AtPME3 that acts as a susceptibility factor and is required for the initial colonization of the host tissue.
细菌或真菌坏死营养型生物产生能够降解果胶的酶的能力通常与侵染过程的成功启动有关。果胶以高度甲酯化的形式合成，随后在壁内被果胶甲酯酶去甲酯化。去酯化使果胶更容易受到坏死营养型病原体在感染初期分泌的果胶酶（如内切多聚半乳糖醛酸酶（endoPG）和果胶裂解酶）的降解。我们表明，在感染过程中，胡萝卜软腐果胶杆菌和灰葡萄孢会在拟南芥中诱导快速表达 AtPME3，AtPME3 作为感病因子发挥作用，并且是宿主组织初始定殖所必需的。
