Salem Dina, Aziz Ahmed Z Abdel
Department of Agricultural Biotechnology, College of Biotechnology, Misr University for Science and Technology, P.O. Box 77, Giza 12566, 6th of October City, Egypt.
Department of Environmental Biotechnology, College of Biotechnology, Misr University for Science and Technology, P.O. Box 77, Giza 12566, 6th of October City, Egypt.
Sci Rep. 2025 Aug 18;15(1):30160. doi: 10.1038/s41598-025-13792-9.
The Exosome-like nanoparticles (ELNs) are essential for both intra- and inter-kingdom communication. The presence and function of plant ELNs discharged into the environment remain unknown, despite their significant biological significance. In the present study, polymer precipitation method was used to isolate the ELNs from Actinidia deliciosa followed by characterization and antifungal activity evaluation against F. oxysporum, F. solani, and B. cinerea in the term of germination and density of fungal spores' formation. Root-released EVs (extracellular vesicles) were found to have a size ranged from 30 to 150 nm, according to biophysical assessments using dynamic light scattering, and transmission electron microscope. Thirty-three unique proteins were identified using LC-MS/MS of ELNs. Several of these proteins are known to be associated with interactions between plants and microbes. The presence of multiple plant defense-related proteins in these EVs raises the possibility that they are important elements of plant immunity.
类外泌体纳米颗粒（ELNs）对于 kingdom 内和 kingdom 间的通讯都至关重要。尽管植物 ELNs 具有重大生物学意义，但其释放到环境中的存在情况及功能仍不为人知。在本研究中，采用聚合物沉淀法从美味猕猴桃中分离 ELNs，随后对其进行表征，并从真菌孢子萌发和形成密度方面评估其对尖孢镰刀菌、茄类镰刀菌和灰葡萄孢的抗真菌活性。根据使用动态光散射和透射电子显微镜进行的生物物理评估，发现根部释放的细胞外囊泡（EVs）大小在 30 至 150 纳米之间。使用 ELNs 的液相色谱 - 串联质谱（LC-MS/MS）鉴定出 33 种独特蛋白质。其中几种蛋白质已知与植物和微生物之间的相互作用有关。这些 EVs 中存在多种植物防御相关蛋白质，这增加了它们是植物免疫重要元素的可能性。
