Chan Ki, Ng Tzi Bun
Biomedical and Tissue Engineering Research Group, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong, China.
Protein Pept Lett. 2011 Apr;18(4):423-33. doi: 10.2174/092986611794653978.
The advent of sugar-immobilized gold nano-particles (SGNPs), lipid-based nanoparticles, nano-chromatography and nano-electrophoresis has revolutionized the methodology for protein purification and proteomic research. This review provides an overview on the effective method developed for fast purification of protein from extracts using SGNPs. In addition, the current application of microfluidic systems for analytical purposes in biochemistry will also be explored that include the micro total analysis systems (µ-TAS) and lab-on-a-chip (LOC) analyses which are capable of isolation and detection of protein at the nanogram level. Finally, we describe why the lipid-based nano-particles (LBNPs) can enable the analysis in microchip electroseparation and how anionic and cationic LBNPs can be used for protein separation.
糖固定化金纳米颗粒（SGNPs）、脂质基纳米颗粒、纳米色谱和纳米电泳的出现彻底改变了蛋白质纯化和蛋白质组学研究的方法。本综述概述了利用SGNPs从提取物中快速纯化蛋白质的有效方法。此外，还将探讨微流控系统目前在生物化学分析中的应用，包括能够在纳克水平分离和检测蛋白质的微全分析系统（µ-TAS）和芯片实验室（LOC）分析。最后，我们阐述了脂质基纳米颗粒（LBNPs）为何能够实现微芯片电分离分析，以及阴离子和阳离子LBNPs如何用于蛋白质分离。
