University of Lausanne, Department of Ecology and Evolution, Quartier Sorge, 1015 Lausanne, Switzerland.
Genome Res. 2011 Mar;21(3):357-63. doi: 10.1101/gr.113803.110. Epub 2010 Dec 20.
We analyze here the relation between alternative splicing and gene duplication in light of recent genomic data, with a focus on the human genome. We show that the previously reported negative correlation between level of alternative splicing and family size no longer holds true. We clarify this pattern and show that it is sufficiently explained by two factors. First, genes progressively gain new splice variants with time. The gain is consistent with a selectively relaxed regime, until purifying selection slows it down as aging genes accumulate a large number of variants. Second, we show that duplication does not lead to a loss of splice forms, but rather that genes with low levels of alternative splicing tend to duplicate more frequently. This leads us to reconsider the role of alternative splicing in duplicate retention.
我们根据最新的基因组数据，分析了选择性剪接和基因复制之间的关系，重点是人类基因组。我们发现，之前报道的选择性剪接水平与家族大小之间的负相关关系不再成立。我们澄清了这种模式，并表明它可以用两个因素来充分解释。首先，随着时间的推移，基因逐渐获得新的剪接变体。这种获得与选择性松弛的状态一致，直到衰老基因积累了大量的变体，从而使净化选择减缓。其次，我们表明，复制不会导致剪接形式的丢失，而是那些选择性剪接水平较低的基因更倾向于频繁复制。这使我们重新考虑选择性剪接在重复保留中的作用。
