From the Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology, HaEmek Medical Centre, Afula, Israel; and Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Obstet Gynecol. 2011 Jan;117(1):55-59. doi: 10.1097/AOG.0b013e31820094f3.
To investigate the effect of a decidual incision on trophoblastic invasion potential in vitro.
Human trophoblast cells were obtained from first-trimester legal terminations of pregnancy. Decidual tissue was retrieved from healthy, low-risk women who underwent an elective cesarean delivery at term. Each dissected decidual sample was divided into four similar-sized samples. The first slice was not treated, the second was incised with a surgical blade to mimic an in vivo injury, the third was incised and immediately repaired with medical adhesive material. This model was used to investigate trophoblastic invasion through a fully repaired decidua. The fourth slice was covered with medical adhesive material only, to exclude any effect of the adhesive material on the decidua. The percent of invasion was calculated as: absorbance of invaded cell×100=invasion index (%). Invasion was expressed as invasion index. The mean and standard deviation of the invasion index were then calculated.
Eight decidual samples were retrieved from eight women. Incised decidua showed a significantly higher mean invasion index (83.3% [±8.1%], P=.012) than the other three models (intact decidua, 69.9% [±5.1%]; incised decidua repaired with adhesive, 66.6% [±8.2%]; intact decidua with adhesive, 58.3% [±11.3%]. There was no significant difference in the invasion index between the other models (P=.4).
Induced decidual injury significantly increased the invasion potential of trophoblastic cells compared with intact decidua. Complete re-approximation of the incised edges reversed this effect in vitro.
研究蜕膜切开对体外滋养细胞侵袭潜能的影响。
从早期妊娠合法终止妊娠的人绒毛滋养细胞中获得人绒毛滋养细胞。从健康、低危的择期行剖宫产术的足月孕妇中获取蜕膜组织。将每个解剖的蜕膜样本分成四个大小相似的样本。第一片未处理，第二片用手术刀片切开以模拟体内损伤，第三片切开后立即用医用粘合材料修复。该模型用于研究通过完全修复的蜕膜进行滋养细胞侵袭。第四片仅用医用粘合材料覆盖，以排除粘合材料对蜕膜的任何影响。侵袭程度按以下公式计算：侵袭细胞的吸光度×100=侵袭指数（%）。侵袭以侵袭指数表示。然后计算侵袭指数的平均值和标准差。
从 8 名妇女中取出了 8 个蜕膜样本。切开的蜕膜显示出明显更高的平均侵袭指数（83.3%[±8.1%]，P=.012），明显高于其他三种模型（完整蜕膜为 69.9%[±5.1%]；用粘合材料修复的切开蜕膜为 66.6%[±8.2%]；用粘合材料覆盖的完整蜕膜为 58.3%[±11.3%]）。其他模型之间的侵袭指数差异无统计学意义（P=.4）。
与完整蜕膜相比，诱导的蜕膜损伤显著增加了滋养细胞的侵袭潜能。在体外，切开边缘的完全重新接近逆转了这种效应。
