果蝇黑腹果蝇含有一种新型的带电荷的脂肪动激素家族肽。
The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide.
作者信息
Schaffer M H, Noyes B E, Slaughter C A, Thorne G C, Gaskell S J
机构信息
Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas 75235-9070.
出版信息
Biochem J. 1990 Jul 15;269(2):315-20. doi: 10.1042/bj2690315.
Abstract
A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.
摘要
在果蝇黑腹果蝇中鉴定出一种节肢动物神经肽RPCH/AKH（红色素浓缩激素/脂肪动激素）家族的成员，并通过自动Edman降解和质谱法，使用快速原子轰击电离和一台具有高灵敏度的串联混合仪器确定了其结构。我们将这种肽称为“DAKH”，其序列为pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2（其中pGlu是焦谷氨酸，Trp-NH2是色氨酸羧酰胺）。对三个身体节段提取物的高效液相色谱分析表明，超过80%的肽存在于胸部。尽管DAKH在其总体结构和在动物体内的分布方面是该家族成员的典型代表，但它独特之处在于含有一个在生理条件下带电荷的残基。文中考虑了这一变化的进化意义。
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