RP-HPLC法测定木通中荷包牡丹苷B的含量
[Determination of calceolarioside B in Caulis Akebiae by RP-HPLC].
作者信息
Gao Hui-Min, Wang Zhi-Min, Qu Li, Fu Xue-Tao, Li Lin
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(6):476-8.
OBJECTIVE
To establish a quantitative method for calceolarioside B in Caulis Akebiae.
METHOD
The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm.
RESULT
The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%.
CONCLUSION
The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.
目的
建立一种定量测定木通中荷包牡丹苷B含量的方法。
方法
采用Phenomenex Luna C18色谱柱，柱温35℃，以甲醇-水-磷酸（35:65:0.05）为流动相进行分离。流速为1.0 mL·min⁻¹，检测波长为330 nm。
结果
校准曲线在0.04至0.86 μg范围内呈线性关系（r = 0.9998），平均回收率为97.2%。对采集自不同地区的39份药材进行测定，木通中荷包牡丹苷B的含量在0.021%至0.745%之间波动。
结论
该方法简便、重复性好，可用于木通的质量控制。
Zotero 插件