Department of Life Science, Shangqiu Normal University, Shangqiu 476000, China.
J Environ Sci (China). 2010;22(9):1418-24. doi: 10.1016/s1001-0742(09)60269-2.
Bacterial abundance and diversity in snow of East Rongbuk, Laohugou and Hailuogou glaciers on the Tibetan Plateau were investigated through epifluorescence microscope and denaturing gradient gel electrophoresis. Cell abundance ranged from 4.0 x 10(3) to 290.2 x 10(3) cells/mL. The phylogenetic trees placed the 16S rRNA sequences in four major groups: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Brevundimonas, Flavobacterium, Hymenobacter, Bacillus, Polaromonas, Rhodoferax and Streptomyces were widely distributed bacteria in glaciers from different cold regions. The remaining five genera of Hylemonella, Delftia, Zoogloea, Blastococcus and Rhodococcus were endemism, only recovered from our investigated glaciers. It is proposed that the three glaciers on the Tibetan Plateau provide a specific ecological niche for prolonging survival of diverse microbial lineages.
通过荧光显微镜和变性梯度凝胶电泳研究了青藏高原东绒布、老沟和海螺沟冰川雪水中的细菌丰度和多样性。细胞丰度范围为 4.0 x 10(3) 至 290.2 x 10(3) 个细胞/ml。系统发育树将 16S rRNA 序列分为四大类：变形菌门、厚壁菌门、放线菌门和拟杆菌门。短小杆菌属、黄杆菌属、海杆菌属、芽孢杆菌属、极性单胞菌属、红杆菌属和链霉菌属是不同寒冷地区冰川中广泛分布的细菌。其余的五个属，Hylemonella、Delftia、Zoogloea、Blastococcus 和 Rhodococcus 是特有种，仅从我们调查的冰川中回收。据提议，青藏高原的这三个冰川为延长不同微生物谱系的生存提供了一个特定的生态位。
