Dunstan R H, ap Rees T, Whatley F R, Greenaway W
Department of Botany, Cambridge, UK.
FEBS Lett. 1990 Aug 20;269(1):177-80. doi: 10.1016/0014-5793(90)81148-h.
Paracoccus denitrificans was grown on either unlabelled glucose, [1-13C]glucose or [6-13C]glucose as the sole carbon source for growth. The cells were then incubated with a range of 14C-glucose substrates to compare the 14CO2-evolution rates between cells grown on the glucose and the 13C-labelled glucose. Cells grown on 13C-glucose had significantly faster rates of 14CO2-evolution than those grown on unlabelled glucose. The % yields of 14CO2, per [1-14C]-, [6-14C]- and [U-14C]glucose supplied were also substantially greater than those measured for cells grown on unlabelled glucose. The data indicated that growth of Paracoccus on 13C-enriched glucose substrates resulted in cells with notably different 14C-glucose oxidation metabolism compared to that observed in cells grown on unlabelled glucose.
反硝化副球菌在未标记的葡萄糖、[1-¹³C]葡萄糖或[6-¹³C]葡萄糖作为唯一碳源的条件下生长。然后将这些细胞与一系列¹⁴C-葡萄糖底物一起孵育，以比较在葡萄糖上生长的细胞和¹³C标记葡萄糖上生长的细胞之间的¹⁴CO₂释放速率。在¹³C-葡萄糖上生长的细胞的¹⁴CO₂释放速率明显快于在未标记葡萄糖上生长的细胞。每供应的[1-¹⁴C]-、[6-¹⁴C]-和[U-¹⁴C]葡萄糖的¹⁴CO₂百分产率也显著高于在未标记葡萄糖上生长的细胞所测得的产率。数据表明，与在未标记葡萄糖上生长的细胞相比，反硝化副球菌在富含¹³C的葡萄糖底物上生长会导致细胞具有明显不同的¹⁴C-葡萄糖氧化代谢。
