Dunstan R H, Whatley F R, Greenaway W
Department of Plant Sciences, University of Oxford, U.K.
Biochem Int. 1988 Apr;16(4):773-83.
Paracoccus denitrificans was grown on [6-13C]-glucose as the sole carbon source for growth and the extracts were fractionated and analysed by gas chromatography-mass spectrometry. The 13C-labelling pattern observed in phenylalanine indicated that the biosynthetic sequences of enzymes for phenylalanine production were unequally distributed within the cell and that there are at least 2 separate loci of phenylalanine biosynthesis. The principal locus of phenylalanine production was associated with the Entner-Doudoroff and/or the pentose phosphate pathways and it was responsible for producing 3/4 of the bacterium's phenylalanine. A second locus was associated with the G6 oxidation pathway and was responsible for producing the remaining 1/4 of the cell's phenylalanine.
反硝化副球菌以[6-¹³C]-葡萄糖作为唯一碳源进行培养，提取物经分级分离后用气相色谱-质谱联用仪进行分析。在苯丙氨酸中观察到的¹³C标记模式表明，用于生产苯丙氨酸的酶的生物合成序列在细胞内分布不均，且至少有2个独立的苯丙氨酸生物合成位点。苯丙氨酸产生的主要位点与恩特纳-杜德洛夫途径和/或磷酸戊糖途径相关，负责产生该细菌3/4的苯丙氨酸。第二个位点与G6氧化途径相关，负责产生细胞其余1/4的苯丙氨酸。
