反硝化副球菌中某些代谢途径定位的稳定同位素证据。
Stable isotope evidence for the localisation of some metabolic pathways in the bacterium Paracoccus denitrificans.
作者信息
Dunstan R H, Whatley F R, Greenaway W
机构信息
Department of Plant Sciences, University of Oxford, U.K.
出版信息
Biochem Int. 1988 Apr;16(4):765-71.
Paracoccus denitrificans was grown on [6-13C]-glucose as the sole carbon source for growth and the extracts were fractionated and analysed by gas chromatography-mass spectrometry. The 13C-enrichments of some metabolites indicated that the "hydrolysate pools" of these metabolites were not in isotopic equilibrium with the water soluble "free pools". It was concluded that localisation of some metabolic pathways had occurred in Paracoccus during growth on [6-13C]-glucose.
反硝化副球菌以[6-¹³C]-葡萄糖作为唯一碳源进行培养，培养后的提取物经过分级分离，并通过气相色谱-质谱联用仪进行分析。一些代谢物的¹³C富集情况表明，这些代谢物的“水解产物池”与水溶性“游离池”并非处于同位素平衡状态。由此得出结论，在以[6-¹³C]-葡萄糖为碳源生长期间，反硝化副球菌中发生了某些代谢途径的定位。
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