Dunstan R H, Whatley F R, Greenaway W
Proc R Soc Lond B Biol Sci. 1987 Aug 21;231(1264):339-47. doi: 10.1098/rspb.1987.0047.
The metabolism of Paracoccus denitrificans, grown on either [2,3-13C]succinate or [1,4-13C]succinate, was investigated by using gas chromatography-mass spectrometry. The distribution of label in a group of metabolites closely related to the TCA-cycle intermediates showed that the flux of carbon from succinate in energy metabolism in vivo was via pyruvate (malic enzyme) and acetyl CoA. The labelling pattern of the carboxyl groups showed that one fifth of the succinate pool was formed by the regeneration of succinate via the TCA cycle, and four fifths was supplied externally as substrate from the medium.
利用气相色谱-质谱联用技术研究了在[2,3-¹³C]琥珀酸或[1,4-¹³C]琥珀酸上生长的反硝化副球菌的代谢情况。一组与三羧酸循环中间体密切相关的代谢物中的标记分布表明，体内能量代谢中琥珀酸的碳通量是通过丙酮酸（苹果酸酶）和乙酰辅酶A进行的。羧基的标记模式表明，五分之一的琥珀酸池是通过三羧酸循环再生琥珀酸形成的，五分之四是作为底物从培养基中外部供应的。
