Department of Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Cancer Sci. 2011 Feb;102(2):446-51. doi: 10.1111/j.1349-7006.2010.01812.x. Epub 2010 Dec 22.
The aim of the present study is to clarify the critical roles of vasohibin in cervical carcinomas. We investigated the expression ratios of vasohibin and vascular endothelial growth factor (VEGF) receptor-2 on endothelium and microvessel density, lymphatic vessel density (LVD) by immunohistochemistry. Sixty-one squamous cell carcinoma (SCC), 18 mucinous adenocarcinoma (Adenocarcinoma), 38 carcinoma in situ (CIS), and 35 normal cervical epithelium were collected. We investigated the expression of vasohibin and compared it with the expression of VEGF receptor-2 (VEGFR-2, KDR/flk-1), and CD34 in the stromal endothelium. Expression of VEGF was counted using the histological score (H score). D2-40 was used as a marker for lymphatic endothelial cells to investigate LVD. The microvessel density of the normal cervical epithelium was significantly lower than that of CIS, SCC, and Adenocarcinoma (P < 0.05). The expression ratio of vasohibin in the normal cervical epithelium was significantly lower than that of SCC and Adenocarcinoma (P < 0.05). The expression ratio of VEGFR-2 of the normal cervical epithelium was significantly lower than that of SCC and Adenocarcinoma (P < 0.05). The LVD of the normal cervical epithelium was significantly lower than that of CIS, SCC, and Adenocarcinoma (P < 0.05). For normal cervical epithelium, CIS, and SCC, there was a moderate correlation between the expression percentage of vasohibin and the expression percentage of VEGFR-2 (P < 0.05, r(2) = 0.3018). This is the first study to elucidate the correlation between the expression of vasohibin in the stromal endothelial cells and the expression of VEGFR-2 in human cervical carcinomas.
本研究旨在阐明血管抑素在宫颈癌中的关键作用。我们通过免疫组织化学方法检测了内皮细胞和微血管密度（MVD）、淋巴管密度（LVD）中血管抑素和血管内皮生长因子（VEGF）受体-2 的表达比值。收集了 61 例鳞状细胞癌（SCC）、18 例黏液腺癌（Adenocarcinoma）、38 例原位癌（CIS）和 35 例正常宫颈上皮组织。我们研究了血管抑素的表达，并将其与血管内皮生长因子受体-2（VEGFR-2，KDR/flk-1）和间质内皮细胞中的 CD34 表达进行了比较。使用组织学评分（H 评分）计算 VEGF 的表达。D2-40 被用作淋巴管内皮细胞的标志物以研究 LVD。正常宫颈上皮组织的 MVD 明显低于 CIS、SCC 和 Adenocarcinoma（P<0.05）。正常宫颈上皮组织中血管抑素的表达比值明显低于 SCC 和 Adenocarcinoma（P<0.05）。正常宫颈上皮组织中 VEGFR-2 的表达比值明显低于 SCC 和 Adenocarcinoma（P<0.05）。正常宫颈上皮组织的 LVD 明显低于 CIS、SCC 和 Adenocarcinoma（P<0.05）。对于正常宫颈上皮、CIS 和 SCC，血管抑素的表达百分比与 VEGFR-2 的表达百分比之间存在中度相关性（P<0.05，r²=0.3018）。这是首次阐明基质内皮细胞中血管抑素表达与人类宫颈癌中 VEGFR-2 表达之间相关性的研究。
