Department of Oral and Maxillofacial Surgery, Shimane University, Faculty of Medicine, Izumo, Japan.
Int J Oral Maxillofac Surg. 2011 Apr;40(4):419-26. doi: 10.1016/j.ijom.2010.10.020. Epub 2010 Dec 19.
This study was designed to investigate the feasibility of using Fas-associated phosphatase-1 (FAP-1), nuclear factor kappa B (NF-κB) and p53 as markers for chemo-radio sensitivity in oral squamous cell carcinoma (OSCC). FAP-1 plays a role as an anti-apoptotic factor through Fas-dependent apoptosis after chemo-radiotherapy. NF-κB and p53 might be involved in modulation of FAP-1 expression. FAP-1, NF-κB and p53 expression were immunohistochemically examined using biopsy specimens in 50 OSCC patients treated with chemotherapy and/or radiotherapy. FAP-1 was expressed in 52%, NF-κB in 52% and p53 in 46% of patients. There was no significant difference in FAP-1, p53 or NF-κB expression according to the clinicopathological features. No correlation was found among FAP-1, p53 or NF-κB expression. FAP-1-positive cases showed a poorer survival rate than FAP-1-negative cases (P = 0.0409) and NF-κB-positive cases showed a poorer survival rate than NF-κB-negative cases (P = 0.0018). Multivariate analysis showed that FAP-1 expression, NF-κB expression, clinical stage and age were significant independent variables for survival (clinical stage: P = 0.0016; age: P = 0.0016; NF-κB: P = 0.0314; FAP-1: P = 0.0366). These results suggest that FAP-1 and NF-κB might play a role as chemo-radioresistant factor during chemo-radiotherapy, and FAP-1 and NF-κB expression in OSCC would be feasible markers for chemo-radio sensitivity and prognosis.
本研究旨在探讨 Fas 相关磷酸酶-1（FAP-1）、核因子 kappa B（NF-κB）和 p53 作为口腔鳞状细胞癌（OSCC）化疗和放疗敏感性标志物的可行性。FAP-1 在化疗和放疗后通过 Fas 依赖性细胞凋亡发挥抗凋亡因子的作用。NF-κB 和 p53 可能参与 FAP-1 表达的调节。使用 50 例接受化疗和/或放疗的 OSCC 患者的活检标本，通过免疫组织化学方法检测 FAP-1、NF-κB 和 p53 的表达。52%的患者表达 FAP-1，52%的患者表达 NF-κB，46%的患者表达 p53。FAP-1、p53 或 NF-κB 的表达与临床病理特征无关。FAP-1、p53 或 NF-κB 之间没有相关性。FAP-1 阳性病例的生存率低于 FAP-1 阴性病例（P=0.0409），NF-κB 阳性病例的生存率低于 NF-κB 阴性病例（P=0.0018）。多因素分析显示，FAP-1 表达、NF-κB 表达、临床分期和年龄是影响生存的独立变量（临床分期：P=0.0016；年龄：P=0.0016；NF-κB：P=0.0314；FAP-1：P=0.0366）。这些结果表明，FAP-1 和 NF-κB 可能在化疗和放疗过程中作为化疗和放疗抵抗因子发挥作用，OSCC 中的 FAP-1 和 NF-κB 表达可能是化疗和放疗敏感性及预后的可行标志物。
