Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 K-Bicêtre, France.
J Clin Microbiol. 2010 May;48(5):1913-4. doi: 10.1128/JCM.02277-09. Epub 2010 Mar 17.
ChromID extended-spectrum beta-lactamase (ESBL) culture medium is routinely used for screening ESBL producers. This medium was tested for detecting carbapenemase-producing Enterobacteriaceae isolates from a collection of reference strains and compared to the CHROMagar KPC culture medium previously evaluated for detecting KPC-producing isolates. Producers of IMP-, VIM-, and KPC-type carbapenemases with high levels of resistance to cephalosporins and to carbapenems were detected at 1x10(1) CFU/ml. The OXA-48 producers were not detected on ChromID ESBL medium unless coexpressing ESBLs, whereas carbapenemase-producing isolates with MICs of <4 microg/ml were not detected on CHROMagar KPC medium.
ChromID 扩展谱β-内酰胺酶(ESBL)培养基通常用于筛选 ESBL 产生菌。该培养基用于检测参考菌株集中的产碳青霉烯酶肠杆菌科分离株,并与先前评估的用于检测产 KPC 分离株的 CHROMagar KPC 培养基进行比较。在 1x10(1)CFU/ml 水平下,可检测到对头孢菌素和碳青霉烯类具有高水平耐药性的 IMP、VIM 和 KPC 型碳青霉烯酶的产生菌。除非同时表达 ESBL,否则无法在 ChromID ESBL 培养基上检测到 OXA-48 产生菌,而在 CHROMagar KPC 培养基上则无法检测到 MIC 值<4μg/ml 的产碳青霉烯酶分离株。