DNA聚合酶α抑制剂对紫外线损伤的HeLa细胞中DNA切除的抑制作用。

Inhibition of DNA excision by DNA polymerase-alpha inhibitor in UV-damaged HeLa cells.

作者信息

Joe C O, Lee M A, Choi B S, Kang K W

机构信息

Department of Biology, Korea Institute of Technology, Taejon.

出版信息

Toxicol Lett. 1990 Aug;52(3):253-9. doi: 10.1016/0378-4274(90)90034-j.

DOI:10.1016/0378-4274(90)90034-j

PMID:2117783

Abstract

HeLa cells treated with 10 J/m2 of ultraviolet (UV) radiation were examined for inhibition of DNA excision by inhibitors of DNA polymerase-alpha and -beta. DNA repair synthesis and excision were inhibited by aphidicolin, a specific inhibitor of DNA polymerase-alpha. Decreased release of radioactive nucleotides from UV-damaged DNA by the inhibitor indicates that the action of DNA polymerase-alpha activity is closely associated with DNA excision. Dideoxythymidine also inhibited DNA repair synthesis but failed to block the excision, suggesting that polymerization by DNA polymerase-beta might precede removal of damaged DNA.

摘要

用10焦耳/平方米紫外线（UV）辐射处理的HeLa细胞，针对DNA聚合酶-α和-β抑制剂对DNA切除的抑制作用进行了检测。阿非迪霉素（一种DNA聚合酶-α的特异性抑制剂）抑制了DNA修复合成和切除。该抑制剂使紫外线损伤的DNA释放的放射性核苷酸减少，这表明DNA聚合酶-α活性的作用与DNA切除密切相关。双脱氧胸苷也抑制了DNA修复合成，但未能阻断切除，这表明DNA聚合酶-β的聚合作用可能先于受损DNA的去除。