Department of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
J Gen Virol. 2011 Apr;92(Pt 4):789-95. doi: 10.1099/vir.0.027854-0. Epub 2010 Dec 22.
To investigate polyomavirus infection in wild rodents, we analysed DNA samples from the spleens of 100 wild rodents from Zambia using a broad-spectrum PCR-based assay. A previously unknown polyomavirus genome was identified in a sample from a multimammate mouse (Mastomys species) and the entire viral genome of 4899 bp was subsequently sequenced. This viral genome contained potential ORFs for the capsid proteins, VP1, VP2 and VP3, and early proteins, small t antigen and large T antigen. Phylogenetic analysis showed that it was a novel member of the family Polyomaviridae, and thus the virus was tentatively named mastomys polyomavirus. After transfection of the viral genome into several mammalian cell lines, transient expression of the VP1 and large T antigen proteins was confirmed by immunoblotting and immunocytochemical analyses. Comparison of large T antigen function in mastomys polyomavirus with that in rhesus monkey polyomavirus SV40 and human polyomavirus JC virus revealed that the large T antigen from mastomys polyomavirus interacted with the tumour suppressor protein pRb, but not with p53.
为了研究野生啮齿动物中的多瘤病毒感染，我们使用广谱 PCR 检测方法分析了来自赞比亚的 100 个野生啮齿动物的脾脏 DNA 样本。在来自多齿滨鼠（Mastomys 属）的样本中发现了一种以前未知的多瘤病毒基因组，并随后对全长为 4899bp 的病毒基因组进行了测序。该病毒基因组包含衣壳蛋白 VP1、VP2 和 VP3，以及早期蛋白小 t 抗原和大 T 抗原的潜在 ORF。系统进化分析表明，它是多瘤病毒科的一个新成员，因此该病毒被暂时命名为多齿滨鼠多瘤病毒。将病毒基因组转染到几种哺乳动物细胞系中后，通过免疫印迹和免疫细胞化学分析证实了 VP1 和大 T 抗原蛋白的瞬时表达。比较多齿滨鼠多瘤病毒与猕猴多瘤病毒 SV40 和人多瘤病毒 JC 病毒的大 T 抗原功能表明，多齿滨鼠多瘤病毒的大 T 抗原与肿瘤抑制蛋白 pRb 相互作用，但不与 p53 相互作用。
