Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
J Gen Virol. 2010 Mar;91(Pt 3):653-8. doi: 10.1099/vir.0.017673-0. Epub 2009 Nov 18.
Serological screening of sera from orang-utans demonstrated a high percentage of sera that cross-reacted with antigens of the polyomavirus (PyV) simian virus 40. Analysis of archival DNA samples from 71 Bornean and eight Sumatran orang-utans with a broad-spectrum PCR assay resulted in the detection of PyV infections in 11 animals from both species. Sequence analysis of the amplicons revealed considerable differences between the PyVs from Bornean and Sumatran orang-utans. The genome from two PyVs, one from each species, was therefore amplified and sequenced. Both viral genomes revealed a characteristic PyV architecture, but lacked an obvious agnogene. Neighbour-joining analysis positioned the viruses in a large cluster together with viruses from bats, bovines, rodents and several primate PyVs from chimpanzees, African green monkeys, squirrel monkeys and the human Merkel cell PyV.
对猩猩血清进行血清学筛查表明，有很大比例的血清与多瘤病毒（PyV）猴病毒 40 的抗原发生交叉反应。使用广谱 PCR 检测方法对来自 71 只婆罗洲猩猩和 8 只苏门答腊猩猩的存档 DNA 样本进行分析，结果在来自两个物种的 11 只动物中检测到了 PyV 感染。对扩增子的序列分析表明，婆罗洲猩猩和苏门答腊猩猩的 PyVs 之间存在相当大的差异。因此，扩增并测序了来自两个物种的两个 PyV 的基因组。这两种病毒的基因组都显示出典型的 PyV 结构，但缺乏明显的 agnogene。邻接法分析将这些病毒与蝙蝠、牛、啮齿动物以及来自黑猩猩、绿长尾猴、松鼠猴和人类 Merkel 细胞 PyV 的几种灵长类 PyV 一起定位在一个大的聚类中。
