来自新大陆灵长类动物的首例多瘤病毒的分子特征：松鼠猴多瘤病毒
Molecular characterization of the first polyomavirus from a New World primate: squirrel monkey polyomavirus.
作者信息
Verschoor Ernst J, Groenewoud Marlous J, Fagrouch Zahra, Kewalapat Aruna, van Gessel Sabine, Kik Marja J L, Heeney Jonathan L
机构信息
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Department of Pathobiology, Pathology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
出版信息
J Gen Virol. 2008 Jan;89(Pt 1):130-137. doi: 10.1099/vir.0.83287-0.
DNA samples from a variety of New World monkeys were screened by using a broad-spectrum PCR targeting the VP1 gene of polyomaviruses. This resulted in the characterization of the first polyomavirus from a New World primate. This virus naturally infects squirrel monkeys (Saimiri sp.) and is provisionally named squirrel monkey polyomavirus (SquiPyV). The complete genome of SquiPyV is 5,075 bp in length, and encodes the small T and large T antigens and the three structural proteins VP1, VP2 and VP3. Interestingly, the late region also encodes a putative agnoprotein, a feature that it shares with other polyomaviruses from humans, baboons and African green monkeys. Comparison with other polyomaviruses revealed limited sequence similarity to any other polyomavirus, and phylogenetic analysis of the VP1 gene confirmed its uniqueness.
使用针对多瘤病毒VP1基因的广谱聚合酶链反应（PCR）对多种新大陆猴的DNA样本进行了筛选。这导致了从新大陆灵长类动物中鉴定出第一种多瘤病毒。这种病毒自然感染松鼠猴（Saimiri sp.），并被临时命名为松鼠猴多瘤病毒（SquiPyV）。SquiPyV的完整基因组长度为5075碱基对，编码小T抗原和大T抗原以及三种结构蛋白VP1、VP2和VP3。有趣的是，晚期区域还编码一种推定的Agno蛋白，这一特征与来自人类、狒狒和非洲绿猴的其他多瘤病毒相同。与其他多瘤病毒的比较显示，与任何其他多瘤病毒的序列相似性有限，并且对VP1基因的系统发育分析证实了其独特性。
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