翼状胬肉中表达的细胞周期蛋白D1与β-连环蛋白的蛋白定位有关。
CyclinD1 protein expressed in pterygia is associated with β-catenin protein localization.
作者信息
Tung Jai-Nien, Chiang Chun-Chi, Tsai Yi-Yu, Chou Ying-Yi, Yeh Kun-Tu, Lee Huei, Cheng Ya-Wen
机构信息
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
出版信息
Mol Vis. 2010 Dec 15;16:2733-8.
BACKGROUND
The Wnt (Wg/Wnt) signaling cascade plays an important role in tumorigenesis. Our previous report indicated that aberrant localization of β-catenin proteins was a feature of pterygia. Therefore, this study aimed to analyze the association of β-catenin protein and expression of a downstream gene, cyclin D1, in pterygial tissues.
METHODS
Using immunohistochemistry, β-catenin and cyclin D1 protein expression was studied, in 150 pterygial specimens and 30 normal conjunctivas.
RESULTS
Seventy-three (48.7%) and 60 (40.0%) pterygial specimens tested positive for β-catenin and cyclin D1 protein expression, respectively. Cyclin D1protein expression was significantly higher in β-catenin-nuclear/cytoplasmic positive groups than in β-catenin membrane positive and negative groups (p < 0.0001). In addition, cyclin D1 expression was significantly higher in the fleshy group than in the atrophic and intermediate groups (p = 0.006).
CONCLUSIONS
Our study demonstrated that β-catenin expressed in nuclei/cytoplasm increases cyclinD1 protein expression, which invokes pterygial cell proliferation.
背景
Wnt（Wg/Wnt）信号级联在肿瘤发生中起重要作用。我们之前的报告表明，β-连环蛋白的异常定位是翼状胬肉的一个特征。因此，本研究旨在分析翼状胬肉组织中β-连环蛋白与下游基因细胞周期蛋白D1表达之间的关联。
方法
采用免疫组织化学方法，研究150例翼状胬肉标本和30例正常结膜中β-连环蛋白和细胞周期蛋白D1的蛋白表达。
结果
分别有73例（48.7%）和60例（40.0%）翼状胬肉标本β-连环蛋白和细胞周期蛋白D1蛋白表达呈阳性。细胞周期蛋白D1蛋白在β-连环蛋白核/细胞质阳性组中的表达显著高于β-连环蛋白膜阳性组和阴性组（p<0.0001）。此外，细胞周期蛋白D1在肥厚型组中的表达显著高于萎缩型组和中间型组（p = 0.006）。
结论
我们的研究表明，细胞核/细胞质中表达的β-连环蛋白会增加细胞周期蛋白D1的蛋白表达，进而引发翼状胬肉细胞增殖。
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