Ding Ai-Shi, Wang Fu-Zhuang, Yu Shun, Wu Li-Ying, Zhao Tong, Fan Ming
Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2002 Feb;18(1):26-9.
To study the effects of hypoxic preconditioning on anoxic tolerance and Jun expression in cultured rat hippocampal neurons after anoxia/reoxygenation.
12 day cultured hippocampal neurons in control and hypoxic preconditioning group were exposed to anoxic environment (0.90L/L N2 + 0.10 L/L CO2) for 4 h, and then reoxygenated for either 24 h or 72 h. The neurons were immunocytochemically stained using the antiserum against Jun. The number of survival neurons and the percentage of Jun expressing neurons were investigated.
The percentage of Jun expressing neurons induced by anoxia in hypoxic-preconditioning group was significantly less than that in control group. The number of survival neurons was more in the hypoxic-preconditioning group than that in control group after anoxic reoxygenation.
Hypoxic-preconditioning can induce the development of anoxic-tolerance in cultured hippocampal neurons. The decrease in Jun expressing neurons in hippocampus may be an adaptive reaction to acute anoxia.
研究缺氧预处理对培养的大鼠海马神经元缺氧复氧后缺氧耐受性及Jun表达的影响。
将对照组和缺氧预处理组培养12天的海马神经元置于缺氧环境（0.90L/L N₂ + 0.10 L/L CO₂）中4小时，然后再复氧24小时或72小时。用抗Jun抗血清对神经元进行免疫细胞化学染色。观察存活神经元数量及Jun表达神经元的百分比。
缺氧预处理组缺氧诱导的Jun表达神经元百分比显著低于对照组。缺氧复氧后，缺氧预处理组存活神经元数量多于对照组。
缺氧预处理可诱导培养的海马神经元产生缺氧耐受性。海马中Jun表达神经元数量的减少可能是对急性缺氧的一种适应性反应。
