Chomwal Rajiv, Kumar Amit, Goyal Anju
Department of Pharmaceutical Chemistry, Swami Keshvanand Institute of Pharmacy, Bikaner 334001, India.
J Pharm Bioallied Sci. 2010 Oct;2(4):365-8. doi: 10.4103/0975-7406.72142.
The study aims to develop simple, sensitive, rapid, accurate and precise spectrophotometric method for estimation of Zolpidem tartrate in tablet dosage forms.
For method I, II, III and IV in a series of 10 ml volumetric flask, aliquots of standard drug solution (100 µg/ml) in 0.1N HCl were transferred and diluted with same so as to give several dilutions in concentration range of 5-30 µg/ml, 5-30 µg/ml, 10-50 µg/ml and 5-40 µg/ml respectively of zolpidem tartrate. To 5 ml of each dilution taken in a separating funnel, (5 ml of bromo phenol red, bromo cresol purple, bromo cresol green and bromo phenol blue for method I, II, III and IV respectively) reagent and 5 ml of chloroform was added. Reaction mixture was shaken gently for 5 min and allowed to stand so as to separate aqueous and chloroform layer. Absorbance maxima measured at 407 nm, 417 nm, 412 nm and 415 nm for method I, II, III and IV respectively.
The recovery studies were found close to 100 % that indicates accuracy and precision of the proposed methods. The statistical analysis was carried out and results of which were found satisfactory. Standard deviation values were found low that indicated reproducibility of the proposed methods.
Based on results the developed methods could be used for routine estimation of zolpidem tartrate from tablet formulations.
本研究旨在开发一种简单、灵敏、快速、准确且精密的分光光度法，用于测定片剂剂型中酒石酸唑吡坦的含量。
对于方法I、II、III和IV，在一系列10毫升容量瓶中，移取0.1N盐酸中的标准药物溶液（100微克/毫升） aliquots，并以相同溶液稀释，以分别得到酒石酸唑吡坦浓度范围为5 - 30微克/毫升、5 - 30微克/毫升、10 - 50微克/毫升和5 - 40微克/毫升的几种稀释液。对于在分液漏斗中取的每种稀释液5毫升，（方法I、II、III和IV分别加入5毫升溴酚红、溴甲酚紫、溴甲酚绿和溴酚蓝）试剂和5毫升氯仿。反应混合物轻轻振荡5分钟，然后静置以分离水相和氯仿层。方法I、II、III和IV分别在407纳米、417纳米、412纳米和415纳米处测量最大吸光度。
回收率研究发现接近100%，表明所提出方法的准确性和精密性。进行了统计分析，结果令人满意。发现标准偏差值较低，表明所提出方法的可重复性。
基于结果，所开发的方法可用于从片剂制剂中常规测定酒石酸唑吡坦。
