Lähdetie J, Räty R, Sorsa M
Department of Medical Genetics, University of Turku, Finland.
Mutat Res. 1990 Sep;245(1):27-32. doi: 10.1016/0165-7992(90)90021-b.
The effects of sodium 2-mercaptoethane sulfonate (Mesna) on the mutagenicity of cyclophosphamide (CP) were assessed in vitro by the Ames test and in vivo in rats by analyzing micronuclei in bone marrow and mutagenic activity in urine. Mesna alone was negative in all test systems, while CP gave a positive response in all of them. In a combined treatment there was no significant reduction of the CP-induced mutagenicity in Salmonella. In rats the frequency of bone marrow micronuclei was not diminished when Mesna was given together with CP. May-Grunwald-Giemsa staining and Hoechst-Pyronin fluorescent staining techniques for micronuclei yielded similar results. The urine of rats treated with CP was mutagenic to Salmonella and no significant difference was observed when the rats had received both Mesna and CP. The results give support to the theory that Mesna acts primarily by reducing the toxicity of metabolites of CP, particularly acrolein, in the urinary tract and not by suppressing the mutagenicity of the active metabolites of CP.
通过艾姆斯试验在体外评估了2-巯基乙烷磺酸钠（美司钠）对环磷酰胺（CP）致突变性的影响，并通过分析大鼠骨髓中的微核和尿液中的诱变活性在体内进行了评估。单独使用美司钠在所有测试系统中均呈阴性，而CP在所有测试系统中均呈阳性反应。在联合治疗中，CP诱导的沙门氏菌致突变性没有显著降低。在大鼠中，当美司钠与CP一起给药时，骨髓微核的频率没有降低。用于微核的May-Grunwald-Giemsa染色和Hoechst-Pyronin荧光染色技术产生了相似的结果。用CP处理的大鼠尿液对沙门氏菌具有诱变性，当大鼠同时接受美司钠和CP时，未观察到显著差异。这些结果支持了这样一种理论，即美司钠主要通过降低CP代谢产物（特别是丙烯醛）在尿路中的毒性起作用，而不是通过抑制CP活性代谢产物的诱变性起作用。
