Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, N7491, Trondheim, Norway.
School of Biotechnology, International University, Vietnam National University, Quarter 6, Linh Trung ward, Thu Duc District, HoChiMinh City, Vietnam.
Sci Rep. 2017 Aug 17;7(1):8611. doi: 10.1038/s41598-017-09073-9.
Little information is available on the link between host development (growth rate and ontogeny) and the composition of the microbiota in fish larvae. This study was carried out to examine potential correlations of microbiota composition with age and growth rate of Atlantic cod larvae. Small and large cod larvae of the same age, representing slow and fast growing individuals, were sampled 10 times during a period of 42 days post hatching (dph), and the composition of the larval microbiota was investigated using a PCR/DGGE (Denaturing Gradient Gel Electrophoresis) strategy. We found significant differences in the intestinal microbiota of small and large larvae of the same age for 4 of the 10 age stages studied. We further found that the variation in the composition of the larval microbiota was more strongly correlated to age than to growth rate for larvae up to 28 dph, whereas for the older larvae growth rate and age was equally correlated to the composition of the microbiota. These results indicate that larval development may structure the microbiota through a change in selection pressure due to host-microbe and microbe-microbe interactions, and that the composition of the microbiota may influence larval development through improved energy gain.
关于宿主发育（生长速度和个体发生）与鱼类幼虫微生物区系组成之间的联系，目前所知甚少。本研究旨在探讨微生物区系组成与大西洋鳕鱼幼虫年龄和生长速度之间的潜在相关性。在孵化后 42 天的一段时间内，10 次采集了同龄的小鳕鱼和大鳕鱼幼虫样本，这些样本代表了生长缓慢和生长迅速的个体，使用 PCR/DGGE（变性梯度凝胶电泳）策略研究了幼虫微生物区系的组成。我们发现，在所研究的 10 个年龄阶段中的 4 个阶段，同龄的小鳕鱼和大鳕鱼幼虫的肠道微生物区系存在显著差异。我们进一步发现，对于 28 日龄以下的幼虫，微生物区系组成的变化与年龄的相关性强于与生长速度的相关性，而对于年龄较大的幼虫，生长速度和年龄与微生物区系的组成同样相关。这些结果表明，幼虫发育可能通过宿主-微生物和微生物-微生物相互作用引起的选择压力的变化来构建微生物区系，并且微生物区系的组成可能通过提高能量获取来影响幼虫发育。
