Chen Kefei, Wei YongGang, Yang HanTeng, Li Bo
Department of Surgery, Division of Liver, West China Hospital, Sichuan University, Chengdu, China.
Genet Test Mol Biomarkers. 2011 Apr;15(4):251-5. doi: 10.1089/gtmb.2010.0208. Epub 2010 Dec 27.
Chronic hepatitis B virus (HBV) infection is a risk factor of hepatocellular carcinoma (HCC) in China. Epidermal growth factor (EGF) plays an important role in tumorigenesis. The association between EGF +61 G/A polymorphism and the risk of HCC is still controversial and ambiguous.
The objective of this study was to investigate the association between EGF +61 G/A polymorphism and the risk of HCC in a Chinese population.
A hospital-based case-control study was designed in a Chinese population. EGF +61 G/A polymorphisms were determined in 120 chronic HBV-infected HCC patients, 120 chronic HBV-infected cirrhotic patients, and 120 healthy controls. The genotype frequency of this polymorphism was determined by using a polymerase chain reaction-restriction fragment length polymorphism assay.
EGF +61 GG (odds ratio=2.76, 95% confidence interval=1.03, 7.38; p=0.04) and G allele frequencies (odds ratio=1.59, 95% confidence interval=1.08, 2.34; p=0.02) in the HCC group were higher than those in the cirrhosis group. EGF +61 A and G allele frequencies in healthy subjects were 28.8% and 71.2%. No relationship between EGF +61 G/A gene polymorphism and HCC risk was found among our recruited HCC patients and healthy controls.
This study suggests that EGF +61 GG genotype is associated with a higher risk of chronic HBV-infected HCC in the Chinese population.
慢性乙型肝炎病毒（HBV）感染是中国肝细胞癌（HCC）的一个危险因素。表皮生长因子（EGF）在肿瘤发生中起重要作用。EGF +61 G/A多态性与HCC风险之间的关联仍存在争议且不明确。
本研究的目的是调查中国人群中EGF +61 G/A多态性与HCC风险之间的关联。
在中国人群中设计了一项基于医院的病例对照研究。对120例慢性HBV感染的HCC患者、120例慢性HBV感染的肝硬化患者和120例健康对照者进行EGF +61 G/A多态性检测。采用聚合酶链反应-限制性片段长度多态性分析方法确定该多态性的基因型频率。
HCC组中EGF +61 GG（比值比=2.76，95%置信区间=1.03，7.38；p=0.04）和G等位基因频率（比值比=1.59，95%置信区间=1.08，2.34；p=0.02）高于肝硬化组。健康受试者中EGF +61 A和G等位基因频率分别为28.8%和71.2%。在我们招募的HCC患者和健康对照者中，未发现EGF +61 G/A基因多态性与HCC风险之间存在关联。
本研究表明，在中国人群中，EGF +61 GG基因型与慢性HBV感染的HCC风险较高相关。
