National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
FEBS Lett. 2011 Jan 21;585(2):435-9. doi: 10.1016/j.febslet.2010.12.036. Epub 2010 Dec 25.
Plant microRNAs (miRNAs) are vital components of the translation control system that regulates plant development and reproduction. The biological function of sly-miR156 was investigated by over-expression in tomato plants. Transgenic tomato plants exhibited a drastically altered phenotype, with reduced height, smaller but more numerous leaves, and smaller fruit. The inflorescence structure of sly-miR156 over-expressing plants phenocopied the sft mutant. The putative targets of sly-miR156 were identified by data base search and included six SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box transcription factor genes. Their expression patterns were then determined in 35S-miR156a and wild type tomato plants. These target genes, as well as the tomato FLOWERING LOCUS T (FT) ortholog SFT, were significantly down-regulated in sly-miR156 over-expressing plants. These studies reveal novel phenotypes regulated by miR156.
植物 microRNAs（miRNAs）是翻译控制系统的重要组成部分，可调节植物的发育和繁殖。通过在番茄植物中过表达 sly-miR156 来研究其生物学功能。转基因番茄植物表现出明显的表型改变，植株变矮，叶片变小但数量增多，果实变小。sly-miR156 过表达植物的花序结构与 sft 突变体相似。通过数据库搜索鉴定了 sly-miR156 的假定靶标，包括六个 SQUAMOSA PROMOTER BINDING PROTEIN（SBP）-box 转录因子基因。然后在 35S-miR156a 和野生型番茄植物中确定了它们的表达模式。这些靶基因以及番茄 FLOWERING LOCUS T（FT）直系同源物 SFT 在 sly-miR156 过表达植物中显著下调。这些研究揭示了由 miR156 调控的新表型。
