Small Animal Clinic and the German Research Foundation SFB Transregio 37, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
Anticancer Res. 2010 Dec;30(12):5043-8.
Canine lymphoma is a commonly occurring, spontaneously developing neoplasia similar to human non-Hodgkin's lymphoma and, thus, is used as a valuable model for human malignancy. HMGB1 and RAGE are strongly associated with tumour progression and vascularisation. Consequently, deregulated RAGE and HMGB1 may play an important role in the mechanisms involved in lymphoma progression.
Expression patterns of HMGB1 and RAGE were analysed in 22 canine lymphoma and three canine non-neoplastic control samples via real time PCR and canine beta-glucuronidase gene (GUSB) as endogenous control.
HMGB1 was up-regulated in the neoplastic samples, while RAGE expression remained inconspicuous.
This study demonstrated similar mechanisms in lymphoma progression in humans and dogs due to overexpression of HMGB1, which was described in human lymphomas. RAGE remained stable in terms of expression indicating that the extracellular HMGB1-induced effects are regulated by HMGB1 itself.
犬淋巴瘤是一种常见的自发性肿瘤，类似于人类非霍奇金淋巴瘤，因此被用作人类恶性肿瘤的有价值的模型。HMGB1 和 RAGE 与肿瘤进展和血管生成密切相关。因此，失调的 RAGE 和 HMGB1 可能在淋巴瘤进展中涉及的机制中发挥重要作用。
通过实时 PCR 和犬β-葡糖苷酸酶基因（GUSB）作为内参，分析了 22 个犬淋巴瘤和 3 个犬非肿瘤对照样本中 HMGB1 和 RAGE 的表达模式。
HMGB1 在肿瘤样本中上调，而 RAGE 表达不明显。
由于 HMGB1 在人类淋巴瘤中被描述为过度表达，本研究证明了人类和犬淋巴瘤进展中存在相似的机制。RAGE 的表达保持稳定，表明细胞外 HMGB1 诱导的效应受 HMGB1 本身的调节。
