Pugliese Michela, Sfacteria Alessandra, Oliva Gaetano, Falcone Annastella, Gizzarelli Manuela, Passantino Annamaria
Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy.
Animals (Basel). 2021 Mar 9;11(3):754. doi: 10.3390/ani11030754.
This study aimed to investigate the role of oxidative stress parameters (ROMs, OXY, SHp), the Oxidative Stress index (OSi), and High Mobility Group Box-1 protein (HMGB-1) in canine leishmaniosis (CanL). For this study, thirty dogs, naturally infected with spp. (Leishmania Group, LEISH) and ten healthy adult dogs (control group, CTR) were included. The diagnosis of CanL was performed by a cytological examination of lymph nodes, real time polymerase chain reaction on biological tissues (lymph nodes and whole blood), and an immunofluorescence antibody test (IFAT) for the detection of anti- antibodies associated with clinical signs such as dermatitis, lymphadenopathy, onychogryphosis, weight loss, cachexia, lameness, conjunctivitis, epistaxis, and hepatosplenomegaly. The HMGB-1 and oxidative stress parameters of the LEISH Group were compared with the values recorded in the CTR group (Mann Whitney Test, < 0.05). Spearman rank correlation was applied to evaluate the correlation between the HMGB-1, oxidative stress biomarkers, hematological and biochemical parameters in the LEISH Group. Results showed statistically significant higher values of SHp in the LEISH Group. Specific correlation between the ROMs and the number of red blood cells, and between HGMB-1 and SHp were recorded. These preliminary data may suggest the potential role of oxidative stress in the pathogenesis of CanL. Further studies are undoubtedly required to evaluate the direct correlation between inflammation parameters with the different stages of CanL. Similarly, further research should investigate the role of ROMs in the onset of anemia.
本研究旨在探讨氧化应激参数（活性氧物质、氧含量、巯基蛋白）、氧化应激指数（OSi）和高迁移率族蛋白B1（HMGB-1）在犬利什曼病（CanL）中的作用。在本研究中，纳入了30只自然感染 spp.（利什曼原虫属组，LEISH）的犬和10只健康成年犬（对照组，CTR）。通过淋巴结细胞学检查、生物组织（淋巴结和全血）的实时聚合酶链反应以及免疫荧光抗体试验（IFAT）对CanL进行诊断，以检测与皮炎、淋巴结病、爪甲肥厚、体重减轻、恶病质、跛行、结膜炎、鼻出血和肝脾肿大等临床症状相关的抗 抗体。将LEISH组的HMGB-1和氧化应激参数与CTR组记录的值进行比较（Mann-Whitney检验， < 0.05）。应用Spearman等级相关性分析评估LEISH组中HMGB-1、氧化应激生物标志物、血液学和生化参数之间的相关性。结果显示，LEISH组中巯基蛋白（SHp）的值在统计学上显著更高。记录到活性氧物质（ROMs）与红细胞数量之间以及高迁移率族蛋白B1（HGMB-1）与巯基蛋白（SHp）之间存在特定相关性。这些初步数据可能提示氧化应激在CanL发病机制中的潜在作用。无疑需要进一步研究来评估炎症参数与CanL不同阶段之间的直接相关性。同样，进一步的研究应调查活性氧物质（ROMs）在贫血发生中的作用。
