Malhotra Ranjan, Grover Vishakha, Kapoor Anoop, Kapur Rupika
Department of Periodontology and Oral Implantology, National Dental College and Hospital, Mohali, Punjab, India.
Indian J Dent Res. 2010 Oct-Dec;21(4):531-6. doi: 10.4103/0970-9290.74209.
The potential of alkaline phosphatase (ALP) as an important diagnostic marker of gingival crevicular fluid (GCF) has been the subject to investigation since 1970. ALP is stored in specific granules and secretory vesicles of the neutrophils and is mainly released during their migration to the site of infection. It is also present in bacteria within dental plaque, osteoblasts and fibroblasts. It has, thus, become important to elucidate whether GCF levels of ALP are potential measures of the inflammatory activity occurring in the adjacent periodontal tissues.
The aim of this study was to assess the total activity of ALP in the GCF collected from healthy sites, sites with gingivitis and with chronic adult periodontitis. An attempt was also made to establish the correlation of ALP activity with plaque index, gingival index, bleeding index and probing depth.
A total of 18 patients were divided into three groups: viz., healthy sites, Group I; gingivitis, Group II; chronic periodontitis, Group III. Clinical parameters like plaque index, bleeding index, gingival index and probing depth were recorded. The ALP level in GCF of all three groups was determined by spectrophotometric analysis.
Total enzyme activity of ALP was significantly higher in periodontitis as compared with that in healthy and gingivitis sites, and was significantly and positively correlated with probing depth.
ALP can be considered as a periodontal disease marker as it can distinguish between healthy and inflamed sites. However, to better define its capacity for periodontal diagnosis, additional longitudinal studies are required.
自1970年以来，碱性磷酸酶（ALP）作为龈沟液（GCF）重要诊断标志物的潜力一直是研究的主题。ALP储存在中性粒细胞的特定颗粒和分泌小泡中，主要在其迁移至感染部位时释放。它也存在于牙菌斑中的细菌、成骨细胞和成纤维细胞中。因此，阐明GCF中ALP水平是否是相邻牙周组织炎症活动的潜在指标变得很重要。
本研究旨在评估从健康部位、牙龈炎部位和慢性成人牙周炎部位收集的GCF中ALP的总活性。还尝试建立ALP活性与菌斑指数、牙龈指数、出血指数和探诊深度之间的相关性。
总共18名患者被分为三组：即，健康部位，第一组；牙龈炎，第二组；慢性牙周炎，第三组。记录菌斑指数、出血指数、牙龈指数和探诊深度等临床参数。通过分光光度分析测定所有三组GCF中的ALP水平。
与健康部位和牙龈炎部位相比，牙周炎中ALP的总酶活性显著更高，并且与探诊深度显著正相关。
由于ALP能够区分健康部位和炎症部位，因此可被视为牙周疾病标志物。然而，为了更好地确定其牙周诊断能力，还需要进行额外的纵向研究。
