Jouvenot M, Pellerin I, Maréchal G, Royez M, Ordener C, Alkhalaf M, Adessi G L
INSERM U 198, Biochimie Hormonale et des Régulations, Besançon, France.
Reprod Nutr Dev. 1990;30(3):455-8. doi: 10.1051/rnd:19900320.
The c-fos expression was investigated in primary culture of guinea pig glandular epithelial cells. These cells were made quiescent by serum deprivation and stimulated with fetal calf serum (FCS, 15%), 17 beta-estradiol (E2 10(-8) mol/l) alone or in combination with epidermal growth factor (EGF, 100 ng/ml) and insulin (I, 10 micrograms/ml). Low levels of c-fos mRNA were detectable in quiescent cells and were not increased in cells stimulated with either E2, EGF, I, or EGF plus I. On the contrary, the c-fos mRNA were early and transiently increased by FCS or E2 plus EGF plus I (4.5 and 9.5 fold induction, respectively). This effect was independent of de novo protein synthesis since it was not abolished in the presence of cycloheximide. It appears that E2 acts in a multiple step process including the stimulation by EGF plus insulin.
在豚鼠腺上皮细胞原代培养物中研究了c-fos的表达。通过血清剥夺使这些细胞静止，并用胎牛血清（FCS，15%）、单独的17β-雌二醇（E2，10(-8) mol/l）或与表皮生长因子（EGF，100 ng/ml）和胰岛素（I，10微克/ml）联合刺激。在静止细胞中可检测到低水平的c-fos mRNA，在用E2、EGF、I或EGF加I刺激的细胞中其水平未升高。相反，FCS或E2加EGF加I可使c-fos mRNA早期短暂增加（分别诱导4.5倍和9.5倍）。这种效应与从头合成蛋白质无关，因为在存在环己酰亚胺的情况下它并未被消除。似乎E2在包括EGF加胰岛素刺激在内的多步骤过程中起作用。
