Yuo A, Miyazono K, Urabe A, Takaku F
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo.
Jpn J Cancer Res. 1990 Aug;81(8):820-6. doi: 10.1111/j.1349-7006.1990.tb02651.x.
We studied granulocyte-macrophage (GM) colony formation in chronic myelomonocytic leukemia (CMML, 6 cases), as compared with that in myelodysplastic syndromes (MDS, 6 cases) and myeloproliferative disorders (MPD, 12 cases). GM colony formation of bone marrow cells by colony-stimulating factor (CSF) was normal in CMML and MPD patients, but was decreased in MDS patients. Circulating granulocyte-macrophage progenitors (CFU-GM) were detected in CMML and MPD patients, but not in MDS patients. GM colony formation without CSF was observed in CMML patients, but not in MDS or MPD patients. These endogenous colonies decreased markedly after adherent cell (AdC) depletion, but AdC did not form endogenous colonies in sufficient numbers to explain their marked decrease after AdC depletion. In CMML patients, the numbers of circulating CFU-GM and endogenous colonies correlated with leukocyte and monocyte counts, respectively. The cellular composition of GM colonies was normal in MDS and MPD patients, whereas granulocytic colonies predominated in all CMML patients but one. The CSF-producing capacity of peripheral blood cells was also studied and was found to be increased in CMML patients. This capacity was markedly decreased by AdC depletion; and AdC could produce CSF only in CMML patients. CSF produced by CMML patients supported granulocytic colonies to a greater extent than CSF produced by MDS or MPD patients. These results suggest that enhanced granulopoiesis in CMML patients is closely associated with the possible hyperproduction of granulocytic CSF by their adherent monocytes.
我们研究了慢性粒单核细胞白血病（CMML，6例）中的粒细胞-巨噬细胞（GM）集落形成，与骨髓增生异常综合征（MDS，6例）和骨髓增殖性疾病（MPD，12例）进行了比较。集落刺激因子（CSF）刺激下骨髓细胞的GM集落在CMML和MPD患者中正常，但在MDS患者中减少。CMML和MPD患者中检测到循环粒细胞-巨噬细胞祖细胞（CFU-GM），而MDS患者中未检测到。CMML患者中观察到无CSF时的GM集落形成，而MDS或MPD患者中未观察到。这些内源性集落在贴壁细胞（AdC）去除后明显减少，但AdC形成的内源性集落数量不足以解释其在AdC去除后的显著减少。在CMML患者中，循环CFU-GM数量和内源性集落数量分别与白细胞和单核细胞计数相关。MDS和MPD患者中GM集落的细胞组成正常，而除1例患者外所有CMML患者中粒细胞集落占主导。还研究了外周血细胞产生CSF的能力，发现CMML患者中该能力增强。该能力在AdC去除后明显降低；且AdC仅能在CMML患者中产生CSF。CMML患者产生的CSF比MDS或MPD患者产生的CSF在更大程度上支持粒细胞集落。这些结果表明，CMML患者中粒细胞生成增强与其贴壁单核细胞可能过度产生粒细胞CSF密切相关。
