Department of Surgery and Integrated Clinic - Division of Periodontics, Dental School of Araçatuba, São Paulo State University, São Paulo, Brazil.
J Periodontol. 2011 Aug;82(8):1206-11. doi: 10.1902/jop.2010.100549. Epub 2010 Dec 28.
Very limited information is available from in vivo studies about whether smoking and/or nicotine affect gingival tissues in the absence of plaque. The purpose of this study is to evaluate the effect of the systemic administration of nicotine in the proliferation and counting of fibroblast-like cells in the gingival tissue of rats.
Thirty adult male Wistar rats were randomly assigned into two groups to receive subcutaneous injections of a saline solution (control group = group C) or nicotine solution (group N; 3 mg/kg) twice a day. The animals were euthanized 37, 44, or 51 days after the first subcutaneous injection. Specimens were routinely processed for serial histologic sections. Five fields of view in the connective tissue adjacent to the gingival epithelium and above the alveolar bone crest of the maxillary first molar were selected for the counting of fibroblast-like cells. Data were statistically analyzed (P <0.05).
The intergroup analysis detected a lower number of fibroblast-like cells in group N compared to group C on days 37 (2.65 ± 1.41 and 6.67 ± 3.25, respectively), 44 (2.70 ± 1.84 and 8.57 ± 2.37, respectively), and 51 (2.09 ± 1.41 and 7.49 ± 2.60, respectively) (P <0.05). The quantification of fibroblast-like cells showed no significant difference (P >0.05) in the intragroup analysis of control and nicotine throughout experimental periods. In the intergroup analysis, group N had reduced proliferating cell nuclear antigen-positive fibroblasts compared to group C in all periods (P <0.05).
The daily systemic administration of nicotine negatively affected, in vivo, the number and proliferation of fibroblast-like cells in the gingival tissue of rats.
关于在没有菌斑的情况下，吸烟和/或尼古丁是否会影响牙龈组织，体内研究提供的信息非常有限。本研究旨在评估尼古丁全身给药对大鼠牙龈组织中成纤维细胞样细胞增殖和计数的影响。
将 30 只成年雄性 Wistar 大鼠随机分为两组，每天两次皮下注射生理盐水（对照组，C 组）或尼古丁溶液（3mg/kg，N 组）。第一次皮下注射后 37、44 或 51 天处死动物。常规处理标本进行连续组织学切片。选择上颌第一磨牙牙龈上皮相邻的结缔组织和牙槽嵴上方的 5 个视野，用于计数成纤维细胞样细胞。对数据进行统计学分析（P<0.05）。
组间分析显示，与 C 组相比，N 组在第 37 天（分别为 2.65±1.41 和 6.67±3.25）、44 天（分别为 2.70±1.84 和 8.57±2.37）和 51 天（分别为 2.09±1.41 和 7.49±2.60）时成纤维细胞样细胞数量较少（P<0.05）。在整个实验期间，C 组和 N 组的成纤维细胞样细胞计数的组内分析无显著差异（P>0.05）。在组间分析中，与 C 组相比，N 组在所有时期增殖细胞核抗原阳性成纤维细胞均减少（P<0.05）。
尼古丁的每日全身给药在体内对大鼠牙龈组织中成纤维细胞样细胞的数量和增殖产生负面影响。
