Mueller Cornelia Katharina, Thorwarth Michael, Schmidt Michelle, Schlegel Karl Andreas, Schultze-Mosgau Stefan
Department of Oral and Maxillofacial Surgery/Plastic Surgery, University Hospital, Jena, Germany.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Dec;112(6):726-36. doi: 10.1016/j.tripleo.2011.01.004. Epub 2011 Mar 26.
An increasing trend toward implantation in complex cases, as well as early loading, is beginning to emerge in dental implantology. Long-term stability of the inserted implants greatly depends on the osseointegration process. Although there are numerous current research efforts aimed at functionalizing implant surfaces, no single factor has proved to be beneficial for osseointegration. The aim of the present study was to investigate whether a combination coating of collagen I and different cytokines enhances osseointegration.
Commercially available titanium implants (Semados S; Bego Implant Systems, Bremen, Germany) were coated with collagen I and either 1 μg or 10 μg of bone morphogenic protein 2, vascular endothelial growth factor 165, basic fibroblast growth factor 2, or a combination of all 3 factors by using the biodot method. Experimental implants (1 pure titanium, 1 collagen I coated and 8 different cytokine coatings) were inserted in the frontal skulls of 9 domestic pigs (10 implants in each animal). Implants were retrieved 2, 4, and 8 weeks after surgery. Samples were subjected to microradiography and immunohistochemistry for collagen I and osteocalcin.
Implant coating with collagen I significantly increased collagen I (P = .028) and osteocalcin (P = .037) expression at the 2-week follow-up and osteocalcin expression (P = .042) as well as the bone implant contact (P = .049) at the 4-week follow-up compared with pure titanium. Additional cytokine coating had no significant effect compared with the collagen I coating.
It can be concluded that collagen I coating enhances osseointegration. However, additional growth factor application has no further beneficial effects.
在牙种植学领域，复杂病例中的种植以及早期负重正呈现出一种日益增长的趋势。植入种植体的长期稳定性在很大程度上取决于骨整合过程。尽管目前有许多研究致力于使种植体表面功能化，但尚未证明单一因素对骨整合有益。本研究的目的是探究I型胶原蛋白与不同细胞因子的联合涂层是否能增强骨整合。
使用生物点涂法，在市售的钛种植体（Semados S；德国不来梅贝格种植系统公司）上涂覆I型胶原蛋白以及1μg或10μg的骨形态发生蛋白2、血管内皮生长因子165、碱性成纤维细胞生长因子2，或这三种因子的组合。将实验性种植体（1个纯钛种植体、1个涂覆I型胶原蛋白的种植体以及8种不同细胞因子涂层的种植体）植入9头家猪的额骨（每头动物植入10个种植体）。术后2周（应为2周、4周和8周）取出种植体。对样本进行显微放射摄影以及针对I型胶原蛋白和骨钙素的免疫组织化学检测。
与纯钛种植体相比，涂覆I型胶原蛋白的种植体在术后2周时I型胶原蛋白（P = 0.028）和骨钙素（P = 0.037）的表达显著增加，在术后4周时骨钙素表达（P = 0.042）以及骨-种植体接触（P = = 0.049）显著增加。与I型胶原蛋白涂层相比，额外的细胞因子涂层没有显著效果。
可以得出结论，I型胶原蛋白涂层可增强骨整合。然而，额外应用生长因子并无进一步的有益效果。 （注：译文中括号内为原文错误，已按正确内容翻译）
