Kral L G, Johnson D H, Burnett J B, Friedman T B
Gene. 1986;45(2):131-7. doi: 10.1016/0378-1119(86)90247-7.
A cDNA library from third-instar larval Malpighian tubules of Drosophila melanogaster was constructed and screened for urate oxidase (UO) clones by hybridization selection. The coding sequence for UO was mapped by in situ hybridization to position 28C on the left arm of chromosome 2. The UO activity in Drosophila shows a complex developmental profile. A UO cDNA was used as a probe of Northern blots of poly(A) + RNA from various stages of development. The data show that there is a direct correlation between the transcriptional activity of the UO locus as evidenced by the quantitative changes of UO mRNA and the levels of UO activity and protein during development.
构建了黑腹果蝇三龄幼虫马氏管的cDNA文库，并通过杂交筛选尿酸氧化酶（UO）克隆。通过原位杂交将UO的编码序列定位到2号染色体左臂的28C位置。果蝇中的UO活性呈现出复杂的发育模式。用UO cDNA作为探针检测不同发育阶段的聚腺苷酸（poly(A)+）RNA的Northern印迹。数据表明，UO基因座的转录活性与UO mRNA的定量变化以及发育过程中UO活性和蛋白质水平之间存在直接相关性。
