Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Bioresour Technol. 2011 Feb;102(4):3900-10. doi: 10.1016/j.biortech.2010.11.126. Epub 2010 Dec 6.
Agro-industrial wastes of cattle dung, vinegar-production residue and rice straw were solid-state fermented by inoculation with Trichoderma harzianum SQR-T037 (SQR-T037) for production of bioorganic fertilizers containing SQR-T037 and 6-pentyl-α-pyrone (6PAP) to control Fusarium wilt of cucumber in a continuously cropped soil. Fermentation days, temperature, inoculum and vinegar-production residue demonstrated significant effects on the SQR-T037 biomass and the yield of 6PAP, based on fractional factorial design. Three optimum conditions for producing the maximum SQR-T037 biomass and 6PAP yield were predicted by central composite design and validated. Bioorganic fertilizer containing 8.46 log(10) ITS copies g(-1) dry weight of SQR-T037 and 1291.73 mg kg(-1) dry weight of 6PAP, and having the highest (p<0.05) biocontrol efficacy, was achieved at 36.7 fermentation days, 25.9°C temperature, 7.6% inoculum content, 41.0% vinegar-production residue, 20.0% rice straw and 39.0% cattle dung. This is a way to offer a high value-added use for agro-industrial wastes.
牛粪、醋糟和稻草农业工业废料经接种哈茨木霉 SQR-T037（SQR-T037）进行固态发酵，生产含有 SQR-T037 和 6-戊基-α-吡喃酮（6PAP）的生物有机肥料，以控制连作土壤中黄瓜枯萎病。基于部分因子设计，发酵天数、温度、接种量和醋糟对 SQR-T037 生物量和 6PAP 产量有显著影响。通过中心复合设计预测并验证了产生最大 SQR-T037 生物量和 6PAP 产量的三个最佳条件。生物有机肥料含有 8.46 log(10) ITS 拷贝 g(-1)干重的 SQR-T037 和 1291.73 mg kg(-1)干重的 6PAP，具有最高（p<0.05）的生物防治效果，在 36.7 天的发酵天数、25.9°C 的温度、7.6%的接种量、41.0%的醋糟、20.0%的稻草和 39.0%的牛粪条件下获得。这是一种为农业工业废料提供高附加值利用的方法。
