Department of Plant Science, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Quebec H9X 3V9.
Mycologia. 2011 May-Jun;103(3):483-93. doi: 10.3852/10-235. Epub 2010 Dec 30.
Knowledge of mycoparasitism has been focused on how antagonists affect pathogens in relation to mechanisms, metabolites and gene expression. Just as microbial antagonists use a diverse arsenal of mechanisms to dominate interactions with hosts, hosts also have diverse responses to counteract antagonism. In this study differential gene expression of eight mycoparasitism-induced genes and eight host-response genes was monitored during in vivo interactions between the mycoparasite Stachybotrys elegans and hyphae and sclerotia of the host, Rhizoctonia solani over 5 d of interaction. Using real time reverse transcription polymerase chain reaction, comparative analyses demonstrated that hyphal and sclerotial structures triggered different expression patterns. These results indicated that multiple regulatory mechanisms might be involved. The high elevated expression of some genes belonging to the mycoparasite and the host suggest that these genes play an important role during the mycoparasitic process and host defense respectively.
关于真菌寄生的知识主要集中在拮抗物如何通过作用机制、代谢物和基因表达来影响病原体。正如微生物拮抗物利用多种机制来主导与宿主的相互作用一样，宿主也有多种反应来对抗拮抗作用。在这项研究中，在生境中，寄生真菌施地鞭毛霉与宿主立枯丝核菌的菌丝和菌核相互作用的 5 天过程中，监测了 8 个真菌寄生诱导基因和 8 个宿主反应基因的差异基因表达。通过实时逆转录聚合酶链反应，比较分析表明，菌丝和菌核结构触发了不同的表达模式。这些结果表明，可能涉及多种调控机制。一些属于寄生真菌和宿主的基因的高表达表明，这些基因在真菌寄生过程和宿主防御中分别发挥重要作用。
