Li Haifeng, Li Fengjiao, Wang Mengyu, Hou Caibo, Jia Feng, Wang Xifeng, Li Mingjun
College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Henan Haochuang Agricultural Technology Group Company Limited, Zhengzhou, 450001, China.
BMC Plant Biol. 2025 Apr 4;25(1):429. doi: 10.1186/s12870-025-06480-3.
Selenium (Se) is an essential trace element that plays a critical role in human tissue formation, metabolism, and physiological functions. However, many individuals worldwide suffer from Se deficiency diseases. This study aims to evaluate the impact of Se-tolerant LF-17 agents and exogenous NaSeO application on the growth, enzyme activity, and metabolic characteristics of rape seedlings. Treatment LF-3 (inoculation of Se-tolerant LF-17 agent and exogenous NaSeO with the soil Se concentration of 5 mg/kg) led to a 38.62% increase in plant height and a 116.7% increase in fresh weight. And the Se-tolerant LF-17 agent in treatment LF-3 also reduced the oxidative stress induced by exogenous NaSeO compared to that of treatment LF-2 (with the same amount exogenous NaSeO only), as evidenced by the lower activities of SOD, POD, and CAT, as well as less content of malondialdehyde. Furthermore, the upregulation of metabolic pathways such as "cuticle, suberine, and wax biosynthesis" "flavonoid biosynthesis," and "terpenoid backbone biosynthesis" enhanced the plant's stress resistance as revealed by non-targeted metabolomics sequencing method. This approach offers promising applications for improving Se bioavailability in crops, mitigating Se toxicity, addressing global Se deficiency challenges and is expected to contribute to fulfilling the Se supplementation needs of the population.
硒（Se）是一种必需的微量元素，在人体组织形成、新陈代谢和生理功能中起着关键作用。然而，全球许多人患有硒缺乏症。本研究旨在评估耐硒LF-17制剂和外源硒酸钠施用对油菜幼苗生长、酶活性和代谢特征的影响。处理LF-3（接种耐硒LF-17制剂和外源硒酸钠，土壤硒浓度为5mg/kg）使株高增加了38.62%，鲜重增加了116.7%。与处理LF-2（仅施用等量外源硒酸钠）相比，处理LF-3中的耐硒LF-17制剂还降低了外源硒酸钠诱导的氧化应激，这通过较低的超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性以及较少丙二醛含量得以证明。此外，通过非靶向代谢组学测序方法发现，“角质、木栓质和蜡质生物合成”“类黄酮生物合成”和“萜类骨架生物合成”等代谢途径的上调增强了植物的抗逆性。这种方法在提高作物中硒的生物有效性、减轻硒毒性、应对全球硒缺乏挑战方面具有广阔的应用前景，有望有助于满足人群的硒补充需求。
