Katakura Masanori, Hashimoto Michio, Tanabe Yoko, Shido Osamu
Department of Environmental Physiology, Shimane University Faculty of Medicine, Izumo, Shimane, 693-8501, Japan.
Med Gas Res. 2012 Jul 9;2(1):18. doi: 10.1186/2045-9912-2-18.
Reactive oxygen species (ROS) production induced by α,β-dicarbonyl compounds and advanced glycation end products causes renal dysfunction in patients with type 2 diabetes and metabolic syndrome. Hydrogen-rich water (HRW) increases the H2 level in blood and tissues, thus reducing oxidative stress in animals as well as humans. In this study, we investigated the effects of HRW on glucose- and α,β-dicarbonyl compound-induced ROS generation in vitro and in vivo.
Kidney homogenates from Wistar rats were incubated in vitro with glucose and α,β-dicarbonyl compounds containing HRW, following which ROS levels were measured. In vivo animal models of metabolic syndrome, SHR.Cg-Leprcp/NDmcr rats, were treated with HRW for 16 weeks, following which renal ROS production and plasma and renal α,β-dicarbonyl compound levels were measured by liquid chromatograph mass spectrometer.
HRW inhibited glucose- and α,β-dicarbonyl compound-induced ROS production in kidney homogenates from Wistar rats in vitro. Furthermore, SHR.Cg-Leprcp/NDmcr rats treated with HRW showed a 34% decrease in ROS production. Moreover, their renal glyoxal, methylglyoxal, and 3-deoxyglucosone levels decreased by 81%, 77%, and 60%, respectively. Positive correlations were found between renal ROS levels and renal glyoxal (r = 0.659, p = 0.008) and methylglyoxal (r = 0.782, p = 0.001) levels.
These results indicate that HRW inhibits the production of α,β-dicarbonyl compounds and ROS in the kidneys of SHR.Cg-Leprcp/NDmcr rats. Therefore, it has therapeutic potential for renal dysfunction in patient with type 2 diabetes and metabolic syndrome.
α，β - 二羰基化合物和晚期糖基化终产物诱导的活性氧（ROS）生成会导致2型糖尿病和代谢综合征患者出现肾功能障碍。富氢水（HRW）可提高血液和组织中的氢气水平，从而减轻动物和人类的氧化应激。在本研究中，我们调查了HRW对体外和体内葡萄糖及α，β - 二羰基化合物诱导的ROS生成的影响。
将Wistar大鼠的肾脏匀浆与含有HRW的葡萄糖和α，β - 二羰基化合物在体外孵育，然后测量ROS水平。对代谢综合征的体内动物模型SHR.Cg - Leprcp/NDmcr大鼠用HRW治疗16周，之后通过液相色谱 - 质谱仪测量肾脏ROS生成以及血浆和肾脏中α，β - 二羰基化合物水平。
HRW在体外抑制了Wistar大鼠肾脏匀浆中葡萄糖和α，β - 二羰基化合物诱导的ROS生成。此外，用HRW治疗的SHR.Cg - Leprcp/NDmcr大鼠的ROS生成减少了34%。而且，它们肾脏中的乙二醛、甲基乙二醛和3 - 脱氧葡萄糖醛酮水平分别下降了81%、77%和60%。发现肾脏ROS水平与肾脏乙二醛（r = 0.659，p = 0.008）和甲基乙二醛（r = 0.782，p = 0.001）水平呈正相关。
这些结果表明，HRW可抑制SHR.Cg - Leprcp/NDmcr大鼠肾脏中α，β - 二羰基化合物和ROS的生成。因此，它对2型糖尿病和代谢综合征患者的肾功能障碍具有治疗潜力。
