Nutrition and Food Science Department, XaRTA, INSA, Pharmacy Faculty, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain.
J Chromatogr A. 2011 Feb 4;1218(5):698-705. doi: 10.1016/j.chroma.2010.12.012. Epub 2010 Dec 13.
Beer is one of the most commonly consumed undistilled alcoholic beverages in many countries. In recent studies, the stilbenes resveratrol and piceid have been found in some hop varieties which are used in the production of beer. Therefore, they could be transferred to beer. The aim of the present work was to validate a method to study the potential content of trans- and cis-resveratrol and piceid in 110 commercial beers from around the world. The resveratrol and piceid contents of 110 beers were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after a solid-phase extraction (SPE) using optimized and validated procedures for the beer matrix. The beer matrix effect was also studied. Stilbenes were found in quantifiable amounts in 92 beers, while concentrations below the limit of quantification (LOQ) were found in 18 beers. Resveratrol was found in the range of 1.34-77.0μg/L in 79% of the beers analyzed, and piceid was found in the range of 1.80-27.3μg/L in only 33% of them. The mean of total resveratrol in all the beers was 14.7±20.5μg/L. The content of resveratrol has been compared with other resveratrol containing foods. A serving of beer contains similar amounts of stilbenes as berries, less than chocolate and grape products but more than pistachios, peanuts or tomatoes. Overall, beer is one of the products with the lowest levels of total resveratrol (μg/L), and despite its high consumption it should not be considered as a representative source of resveratrol.
啤酒是许多国家最常饮用的未蒸馏酒精饮料之一。在最近的研究中，一些用于生产啤酒的啤酒花品种中发现了白藜芦醇和白皮杉醇。因此，它们可能会转移到啤酒中。本研究的目的是验证一种方法，以研究来自世界各地的 110 种商业啤酒中反式和顺式白藜芦醇和白皮杉醇的潜在含量。使用优化和验证的啤酒基质固相萃取（SPE）程序，通过液相色谱-串联质谱（LC-MS/MS）分析了 110 种啤酒中的白藜芦醇和白皮杉醇含量。还研究了啤酒基质效应。在 92 种啤酒中可以定量检测到白藜芦醇和白皮杉醇，而在 18 种啤酒中则低于定量限（LOQ）。在分析的 79％的啤酒中，白藜芦醇的浓度范围为 1.34-77.0μg/L，而只有 33％的啤酒中发现了白皮杉醇的浓度范围为 1.80-27.3μg/L。所有啤酒中总白藜芦醇的平均值为 14.7±20.5μg/L。将白藜芦醇的含量与其他含有白藜芦醇的食物进行了比较。一份啤酒中的白藜芦醇含量与浆果相似，少于巧克力和葡萄产品，但多于开心果，花生或西红柿。总体而言，啤酒是总白藜芦醇含量（μg/L）最低的产品之一，尽管其消费量很高，但不应将其视为白藜芦醇的代表性来源。
