Usman Tahir, Wang Yachun, Liu Chao, He Yanghua, Wang Xiao, Dong Yichun, Wu Hongjun, Liu Airong, Yu Ying
Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Mardan, 23200 Pakistan.
J Anim Sci Biotechnol. 2017 Jan 13;8:5. doi: 10.1186/s40104-016-0137-1. eCollection 2017.
Bovine mastitis is the most common and costly disease of lactating cattle worldwide. Apart from milk somatic cell count (SCC) and somatic cell score (SCS), serum cytokines such as interleukin-17 (IL-17) and interleukin-4 (IL-4) may also be potential indicators for bovine mastitis. The present study was designed to investigate the effects of single nucleotide polymorphisms (SNPs) in bovine - and - genes on SCC, SCS and serum cytokines in Chinese Holstein and Inner-Mongolia Sanhe cattle, and to compare the mRNA expression variations of the cows with different genotypes.
A total of 464 lactating cows (337 Holstein and 127 Inner-Mongolia Sanhe cattle) were screened for SNPs identification and the data were analyzed using fixed effects of herd, parity, season and year of calving by general linear model procedure. The results revealed that SNP g.24392436C > T in - and SNP g.24345410A > G in - showed significant effects on SCC and IL-4 in Holstein ( = 337) and on IL-17 and IL-4 in Sanhe cattle ( = 127). The homozygous GG genotype of SNP g.24345410A > G had significantly higher mRNA expression compared with the heterozygous AG genotype.
The results indicate that - and - could be powerful candidate genes of mastitis resistance and the significant SNPs might be useful genetic markers against mastitis in both dairy and dual purpose cattle.
奶牛乳腺炎是全球泌乳奶牛中最常见且成本最高的疾病。除了乳体细胞计数（SCC）和体细胞评分（SCS）外，血清细胞因子如白细胞介素 - 17（IL - 17）和白细胞介素 - 4（IL - 4）也可能是奶牛乳腺炎的潜在指标。本研究旨在调查中国荷斯坦牛和内蒙古三河牛中 - 和 - 基因单核苷酸多态性（SNP）对SCC、SCS和血清细胞因子的影响，并比较不同基因型奶牛的mRNA表达差异。
共筛选了464头泌乳奶牛（337头荷斯坦牛和127头内蒙古三河牛）进行SNP鉴定，并通过一般线性模型程序使用牛群、胎次、季节和产犊年份的固定效应分析数据。结果显示， - 基因中的SNP g.24392436C>T和 - 基因中的SNP g.24345410A>G对荷斯坦牛（n = 337）的SCC和IL - 4以及三河牛（n = 127）的IL - 17和IL - 4有显著影响。SNP g.24345410A>G的纯合GG基因型与杂合AG基因型相比，mRNA表达显著更高。
结果表明， - 和 - 可能是抗乳腺炎的有力候选基因，显著的SNP可能是奶牛和兼用型牛抗乳腺炎的有用遗传标记。
