Ortíz-Ortíz L, Capin N R, Capin R, Zamacona G
Arch Invest Med (Mex). 1978;9 Suppl 1:351-6.
A passive hemagglutination method useful for the determination of antibodies against Entamoeba histolytica is described. The E. histolytica antigen is attached to group "O" human red blood cells by means of CrCl3. The specificity and sensitivity of the CrCl3 method was compared with other hemagglutination methods, i.e., tannic acid method and a commercial preparation, as well as counterimmunoelectrophoresis. The CrCl3 method showed a reactivity similar to the tannic acid hemagglutination technique and greater specificity than the commercial preparation. The assay reported here showed a good correlation with the counterimmunoelectrophoresis method. The sensitization of erythrocytes with the amebic antigen by means of CrCl3 offers an easy, rapid and specific technique which can be used as a routine test for the detection of antibodies against E. histolytica.
本文描述了一种用于测定抗溶组织内阿米巴抗体的被动血凝方法。溶组织内阿米巴抗原通过三氯化铬附着于“O”型人红细胞。将三氯化铬方法的特异性和敏感性与其他血凝方法（即鞣酸方法和一种商业制剂）以及对流免疫电泳进行了比较。三氯化铬方法显示出与鞣酸血凝技术相似的反应性，且比商业制剂具有更高的特异性。本文报道的检测方法与对流免疫电泳方法具有良好的相关性。通过三氯化铬用阿米巴抗原致敏红细胞提供了一种简便、快速且特异的技术，可作为检测抗溶组织内阿米巴抗体的常规试验。
