利用光纤光谱测量技术定量测量与组织光学特性无关的体内荧光。
Quantification of in vivo fluorescence decoupled from the effects of tissue optical properties using fiber-optic spectroscopy measurements.
机构信息
University of Toronto, Department of Medical Biophysics, Toronto, Ontario, Canada.
出版信息
J Biomed Opt. 2010 Nov-Dec;15(6):067006. doi: 10.1117/1.3523616.
Abstract
We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with the objective of accurately quantifying the fluorescence in absolute units. The primary motivation is measurement of 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) concentration in tissue during fluorescence-guided resection of malignant brain tumors. This technique is validated in phantoms and ex vivo mouse tissues, and tested in vivo in a rabbit brain tumor model using ALA-PpIX fluorescence contrast.
摘要
我们提出了一种使用手持式光纤探头进行组织荧光定量的方法，该探头同时测量荧光和漫反射光谱。我们开发了一种简化方法来分离荧光光谱与组织光学吸收和散射的干扰，目的是准确地以绝对单位定量荧光。主要动机是在荧光引导切除恶性脑肿瘤期间测量组织中的 5-氨基酮戊酸诱导的原卟啉 IX（ALA-PpIX）浓度。该技术在体模和离体小鼠组织中得到验证，并在使用 ALA-PpIX 荧光对比的兔脑肿瘤模型中进行了体内测试。
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