Shafaa Medhat W, El Shazly Laila H, El Shazly Amany H, El gohary Amal A, El hossary Ghada G
Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt.
Vet Ophthalmol. 2011 Jan;14(1):18-25. doi: 10.1111/j.1463-5224.2010.00834.x.
To evaluate the effects of liposome-bound tetracycline eye drops in a rabbit dry eye model evaluating their advantage of being less allergic, preservative free and prolonged action compared with other tear substitutes.
New Zealand albino rabbits were equally divided into control group and dry eye induced groups. Dryness was induced in 24 eyes of 12 healthy adult male albino rabbits by instilling atropine sulfate eye drops 1% three times daily for 1 week, then animals were subdivided into four groups; group 1 (rabbits with dry eye model), groups 2, 3, and 4: rabbits with dry eye model treated for 7 days starting on 7th day of dryness induction with either tetracycline, empty liposome, or combined tetracycline with liposome as topical eye drops respectively. Schirmer (STT) test and tear break up time (TBUT) were assessed on days 0, 2, 4, 7, 9, 11, and 14. Animals were sacrificed on day 14 and histopathological examination of the cornea and conjunctiva was performed.
Tear break up time and STT test values were significantly improved in groups 2, 3, 4 as compared with group 1. The histopathological examination showed normal cytoarchitecture of corneas and conjunctivae in groups 2, 3, 4 against the dryness effect that continued to affect the cornea and conjunctival epithelium in group 1. There was a significant improvement in the group treated with liposome-bound tetracycline eye drops (group 4) as compared with tetracycline alone (group 2) and empty liposome (group 3).
The use of liposome encapsulated tetracycline significantly improved STT and TBUT values as well as reverse surface ocular pathology.
在兔干眼模型中评估脂质体包裹的四环素滴眼液的效果，比较其与其他泪液替代物相比在低过敏性、无防腐剂和长效作用方面的优势。
将新西兰白化兔平均分为对照组和干眼诱导组。通过每天3次滴入1%硫酸阿托品滴眼液，连续1周，诱导12只健康成年雄性白化兔的24只眼睛产生干燥，然后将动物再细分为四组；第1组（干眼模型兔），第2、3、4组：从干燥诱导第7天开始，分别用四环素、空脂质体或脂质体联合四环素作为局部滴眼液，对干眼模型兔进行7天治疗。在第0、2、4、7、9、11和14天评估泪液分泌试验（STT）和泪膜破裂时间（TBUT）。在第14天处死动物，对角膜和结膜进行组织病理学检查。
与第1组相比，第2、3、4组的泪膜破裂时间和STT试验值显著改善。组织病理学检查显示，第2、3、4组的角膜和结膜细胞结构正常，而第1组的角膜和结膜上皮仍受干燥影响。与单独使用四环素（第2组）和空脂质体（第3组）相比，脂质体包裹的四环素滴眼液治疗组（第4组）有显著改善。
使用脂质体包裹的四环素可显著提高STT和TBUT值，并逆转眼表病理状况。
