• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

eMAG™与NucliSENS EasyMAG在临床标本上的性能比较。

Comparison of eMAG™ versus NucliSENS EasyMAG performance on clinical specimens.

作者信息

Garcia Magali, Chessa Céline, Bourgoin Anne, Giraudeau Geneviève, Plouzeau Chloé, Agius Gérard, Lévêque Nicolas, Beby-Defaux Agnès

机构信息

Laboratoire de Virologie et de Mycobactériologie, CHU de Poitiers, Poitiers, France; EA4331-LITEC, Université de Poitiers, Poitiers, France.

Laboratoire de Virologie et de Mycobactériologie, CHU de Poitiers, Poitiers, France.

出版信息

J Clin Virol. 2017 Mar;88:52-57. doi: 10.1016/j.jcv.2017.01.004. Epub 2017 Jan 29.

DOI:10.1016/j.jcv.2017.01.004
PMID:28160729
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7185493/
Abstract

BACKGROUND

eMAG™ (bioMerieux) is a new nucleic acid extraction platform based on magnetic silica technology, like its predecessor, NucliSENS easyMAG (bioMerieux). Using the same reagents and disposables, eMAG™ adds further automation, allowing simultaneous extraction of 48 samples directly from primary tubes, and distribution of nucleic acid extracts on PCR strips or in tubes at the end of the extraction process.

OBJECTIVE

To compare the performance of eMAG™ and easyMAG on various clinical specimens.

STUDY DESIGN

Respiratory (n=199), whole blood (n=50), plasma (n=25) and urine (n=25) specimens were extracted in parallel on both platforms. Both qualitative (respiratory virus, cell control, CMV, EBV, HHV6 and BKV detection) and quantitative (respiratory virus and cell control cycle thresolds, and CMV, EBV, HHV6 and BKV viral loads) results were compared.

RESULTS

Detection of qualitative targets showed good agreement, ranging from 84.6% for whole blood to 95.9% for respiratory specimens. Correlations between quantitative results were good, with R ranging from 0.802 to 0.995. Quantitative results showed average overall differences below 0.10 log copies/mL between eMAG™ and easyMAG.

CONCLUSIONS

The two platforms showed comparable performance on the types of clinical specimen tested. With higher automation and throughput than easyMAG, the eMAG™ platform is likely to be advantageous for laboratories performing a large number of molecular analyses.

摘要

背景

eMAG™（生物梅里埃公司）是一种基于磁性硅胶技术的新型核酸提取平台，与其前身NucliSENS easyMAG（生物梅里埃公司）类似。使用相同的试剂和一次性耗材，eMAG™进一步提高了自动化程度，可直接从原始试管中同时提取48个样本，并在提取过程结束时将核酸提取物分配到PCR条带上或试管中。

目的

比较eMAG™和easyMAG在各种临床标本上的性能。

研究设计

在两个平台上并行提取呼吸道标本（n = 199）、全血标本（n = 50）、血浆标本（n = 25）和尿液标本（n = 25）。比较定性结果（呼吸道病毒、细胞对照、巨细胞病毒、EB病毒、HHV6和BK病毒检测）和定量结果（呼吸道病毒和细胞对照的循环阈值，以及巨细胞病毒、EB病毒、HHV6和BK病毒载量）。

结果

定性指标检测显示一致性良好，全血标本的一致性为84.6%，呼吸道标本为95.9%。定量结果之间的相关性良好，R值范围为0.802至0.995。定量结果显示，eMAG™和easyMAG之间的平均总体差异低于0.10 log拷贝/mL。

结论

在测试的临床标本类型上，这两个平台表现出相当的性能。eMAG™平台具有比easyMAG更高的自动化程度和通量，对于进行大量分子分析的实验室可能具有优势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2f3/7185493/52856cb16228/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2f3/7185493/46c6f35868cd/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2f3/7185493/d4dd74f03daf/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2f3/7185493/52856cb16228/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2f3/7185493/46c6f35868cd/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2f3/7185493/d4dd74f03daf/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2f3/7185493/52856cb16228/gr3_lrg.jpg

相似文献

1
Comparison of eMAG™ versus NucliSENS EasyMAG performance on clinical specimens.eMAG™与NucliSENS EasyMAG在临床标本上的性能比较。
J Clin Virol. 2017 Mar;88:52-57. doi: 10.1016/j.jcv.2017.01.004. Epub 2017 Jan 29.
2
Comparative evaluation of the QIAGEN QIAsymphony® SP system and bioMérieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory.QIAGEN QIAsymphony SP 系统与 bioMérieux NucliSens easyMAG 自动化提取平台在临床病毒学实验室的比较评估。
J Clin Virol. 2011 Dec;52(4):339-43. doi: 10.1016/j.jcv.2011.08.016. Epub 2011 Oct 20.
3
Comparative evaluation of two automated systems for nucleic acid extraction of BK virus: NucliSens easyMAG versus BioRobot MDx.两种 BK 病毒核酸提取自动化系统的比较评估：NucliSens easyMAG 与 BioRobot MDx。
J Virol Methods. 2009 Dec;162(1-2):208-12. doi: 10.1016/j.jviromet.2009.08.007. Epub 2009 Aug 25.
4
Comparison of two nucleic acid extraction and testing systems for HCMV-DNA detection and quantitation on whole blood specimens from transplant patients.比较两种核酸提取和检测系统，用于检测和定量移植患者全血标本中的 HCMV-DNA。
J Virol Methods. 2013 Nov;193(2):579-82. doi: 10.1016/j.jviromet.2013.07.042. Epub 2013 Aug 4.
5
Evaluation of a new extraction platform in combination with molecular assays useful for monitoring immunosuppressed patients.评估一种新的提取平台与分子检测方法联合应用于监测免疫抑制患者的效果。
J Clin Virol. 2018 Nov;108:59-63. doi: 10.1016/j.jcv.2018.09.006. Epub 2018 Sep 15.
6
Performance evaluation of the automated NucliSens easyMAG nucleic acid extraction platform in comparison with QIAamp Mini kit from clinical specimens.与QIAamp Mini试剂盒相比，对临床样本中自动化NucliSens easyMAG核酸提取平台的性能评估。
Diagn Microbiol Infect Dis. 2009 Jun;64(2):158-65. doi: 10.1016/j.diagmicrobio.2009.02.013.
7
Comparative evaluation of the QIAsymphony RGQ system with the easyMAG/R-gene combination for the quantitation of cytomegalovirus DNA load in whole blood.比较 QIAsymphony RGQ 系统与 easyMAG/R-gene 组合在全血中定量检测巨细胞病毒 DNA 载量的效果。
Virol J. 2012 Oct 9;9:231. doi: 10.1186/1743-422X-9-231.
8
Evaluation of NucliSens easyMAG for automated nucleic acid extraction from various clinical specimens.评估NucliSens easyMAG用于从各种临床标本中自动提取核酸的性能。
J Clin Microbiol. 2007 Feb;45(2):421-5. doi: 10.1128/JCM.00894-06. Epub 2006 Dec 13.
9
Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens.采用常规临床呼吸道标本对新型自动化核酸提取系统的前瞻性评估。
J Med Virol. 2012 Jun;84(6):906-11. doi: 10.1002/jmv.23281.
10
Extraction of viral nucleic acids: comparison of five automated nucleic acid extraction platforms.病毒核酸提取：五种自动化核酸提取平台的比较。
J Clin Virol. 2012 Jul;54(3):255-9. doi: 10.1016/j.jcv.2012.03.008. Epub 2012 Apr 13.

引用本文的文献

1
Shaken, not stirred: magnetic bead DNA extraction as a rapid and effective method for the scaling up of bovine tuberculosis diagnosis.摇晃而非搅拌：磁珠DNA提取作为一种快速有效的方法用于扩大牛结核病诊断规模
BMC Vet Res. 2024 Dec 19;20(1):568. doi: 10.1186/s12917-024-04427-8.
2
SARS-CoV-2 Variants May Affect Saliva RT-PCR Assay Sensitivity.SARS-CoV-2 变异株可能影响唾液 RT-PCR 检测的敏感性。
J Appl Lab Med. 2024 Nov 4;9(6):927-937. doi: 10.1093/jalm/jfae095.
3
Viral infection in chronic otitis media with effusion in children.儿童分泌性中耳炎中的病毒感染

本文引用的文献

1
Clinical application of viral cerebrospinal fluid PCR testing for diagnosis of central nervous system disorders: a retrospective 11-year experience.病毒脑脊液PCR检测在中枢神经系统疾病诊断中的临床应用：一项为期11年的回顾性研究经验
Diagn Microbiol Infect Dis. 2014 Nov;80(3):207-15. doi: 10.1016/j.diagmicrobio.2014.07.010. Epub 2014 Aug 6.
2
Comparative evaluation of the QIAsymphony RGQ system with the easyMAG/R-gene combination for the quantitation of cytomegalovirus DNA load in whole blood.比较 QIAsymphony RGQ 系统与 easyMAG/R-gene 组合在全血中定量检测巨细胞病毒 DNA 载量的效果。
Virol J. 2012 Oct 9;9:231. doi: 10.1186/1743-422X-9-231.
3
Front Pediatr. 2023 May 10;11:1124567. doi: 10.3389/fped.2023.1124567. eCollection 2023.
4
Infant deaths from respiratory syncytial virus in Lusaka, Zambia from the ZPRIME study: a 3-year, systematic, post-mortem surveillance project.赞比亚卢萨卡 ZPRIME 研究：一项为期 3 年的系统尸检监测项目，导致呼吸道合胞病毒的婴儿死亡。
Lancet Glob Health. 2022 Feb;10(2):e269-e277. doi: 10.1016/S2214-109X(21)00518-0.
5
Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses.用于呼吸道病毒宏基因组学分析的核酸提取方法比较
Microorganisms. 2020 Oct 6;8(10):1539. doi: 10.3390/microorganisms8101539.
6
Comparison of the new fully automated extraction platform eMAG to the MagNA PURE 96 and the well-established easyMAG for detection of common human respiratory viruses.比较新型全自动提取平台 eMAG 与 MagNA PURE 96 和成熟的 easyMAG 在检测常见人类呼吸道病毒方面的性能。
PLoS One. 2019 Feb 19;14(2):e0211079. doi: 10.1371/journal.pone.0211079. eCollection 2019.
Extraction of viral nucleic acids: comparison of five automated nucleic acid extraction platforms.
病毒核酸提取：五种自动化核酸提取平台的比较。
J Clin Virol. 2012 Jul;54(3):255-9. doi: 10.1016/j.jcv.2012.03.008. Epub 2012 Apr 13.
4
Comparative evaluation of the QIAGEN QIAsymphony® SP system and bioMérieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory.QIAGEN QIAsymphony SP 系统与 bioMérieux NucliSens easyMAG 自动化提取平台在临床病毒学实验室的比较评估。
J Clin Virol. 2011 Dec;52(4):339-43. doi: 10.1016/j.jcv.2011.08.016. Epub 2011 Oct 20.
5
Multiplex PCR and emerging technologies for the detection of respiratory pathogens.多重 PCR 和新兴技术在呼吸道病原体检测中的应用。
Clin Infect Dis. 2011 May;52 Suppl 4(Suppl 4):S326-30. doi: 10.1093/cid/cir047.
6
Comparison of the QIAsymphony automated nucleic acid extraction and PCR setup platforms with NucliSens easyMAG and Corbett CAS-1200 liquid handling station for the detection of enteric pathogens in fecal samples.比较 QIAsymphony 自动化核酸提取和 PCR 设定平台与 NucliSens easyMAG 和 Corbett CAS-1200 液体处理工作站，用于检测粪便样本中的肠道病原体。
J Microbiol Methods. 2011 Feb;84(2):335-40. doi: 10.1016/j.mimet.2010.12.024. Epub 2010 Dec 31.
7
Comparison of commercial systems for extraction of nucleic acids from DNA/RNA respiratory pathogens.商业系统从 DNA/RNA 呼吸道病原体中提取核酸的比较。
J Virol Methods. 2011 Jan;171(1):195-9. doi: 10.1016/j.jviromet.2010.10.024. Epub 2010 Oct 27.
8
Comparative evaluation of two automated systems for nucleic acid extraction of BK virus: NucliSens easyMAG versus BioRobot MDx.两种 BK 病毒核酸提取自动化系统的比较评估：NucliSens easyMAG 与 BioRobot MDx。
J Virol Methods. 2009 Dec;162(1-2):208-12. doi: 10.1016/j.jviromet.2009.08.007. Epub 2009 Aug 25.
9
Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of epstein-barr virus and cytomegalovirus load.商业化全血病毒 DNA 提取自动化系统的比较评估：用于监测 Epstein-Barr 病毒和巨细胞病毒载量的应用。
J Clin Microbiol. 2009 Nov;47(11):3753-5. doi: 10.1128/JCM.01497-09. Epub 2009 Aug 26.
10
Performance evaluation of the automated NucliSens easyMAG nucleic acid extraction platform in comparison with QIAamp Mini kit from clinical specimens.与QIAamp Mini试剂盒相比，对临床样本中自动化NucliSens easyMAG核酸提取平台的性能评估。
Diagn Microbiol Infect Dis. 2009 Jun;64(2):158-65. doi: 10.1016/j.diagmicrobio.2009.02.013.